lab 3 Flashcards
1
Q
one of basic tools of modern biotechnology
A
DNA splicing
2
Q
what procedure are we using in this lab
A
gel electrophoresis
3
Q
are DNA fragments negatively or positively charged
A
negatively
4
Q
what percent agarose gel in TAE are we using
A
1%
5
Q
TAE
A
biological buffer that keeps the pH of the solution in a specific range
- Tris, Acetate, EDTA
6
Q
what are we using to insert DNA sample into gel
A
micropipette
7
Q
L microtube composition
A
uncut lambda DNA
8
Q
P microtube composition
A
Pstl restriction digest of lambda DNA
9
Q
E microtube composition
A
EcoRI restriction digest of lambda DNA
10
Q
H microtube composition
A
HindIII restriction digest of lambda DNA
11
Q
what bacteriophage will be used
A
lambda
12
Q
what DNA stain/dye will we be using
A
UView
13
Q
UView composition
A
- Bromphenol Blue
- Xylene Cylanol
- proprietary fluorescent DNA stain
