hw questions Flashcards
which DNA mutagen results in a transversion mutation
oxygen radicals
which DNA mutagen results in a frameshift mutation
acridine orange
in its common form, the keto form, 5-bromodeoxyuridine is an analog of what nucleotide?
thymine
in drosophila, which cross results in hybrid dysgenesis
P cypotype males to M cyptopye females
what is the transposable elements that acts as a control element in maize?
Ds
Ac elements in yeast contain which gene?
transposase
the transposable element that is the cause of the X-linked blood-clotting disorder hemophilia A is a
LINE element
all bacterial transposable elements have a gene that encodes which of the following enzymes?
transposase
which hereditary condition is a consequence of insertional inactivation?
hemophilia A
what are tautomers of nucleotide bases
isomers that differ from each other in the location of one hydrogen atom in the molecule
what nucleotide will base-pair with the enol form of 5-bromouracil?
guanine
purpose of the Ames test
test the mutagenic effects of chemicals
in the Ames test, the appearance of his+ revertants in the presence of a non-mutagenic control compound indicates that
some of the reversion mutations aren’t caused by the mutagen being tested
true or false: many chemicals are more mutagenic after being processed in the liver
true
true or false: all compounds that have been found to be mutagenic in the Ames test are also carcinogenic
false
why are liver extracts used in the Ames test?
liver enzymes may activate some innocuous compounds, making them mutagenic
which bacteria grow on the agar plate if the Ames test is positive?
his+ prototrophs
to use RFLP analysis to detect a SNP, the SNP must
occur within a restriction enzyme recognition sequence
molecular tests for Huntingtons, cystic fibrosis, and tay-sachs are possible because…
sufficient info gathered on DNA sequences of both the mutant and normal alleles
what is 5BU best described as
a base analog that can cause either A-T > G-C or G-C > A-T transitions
for 5BU to cause a transition mutation, which of the following must occur
DNA with incorporated 5BU must replicate
true or false: in its rare form, 5BU pairs with guanine
true
what are the consequences of having pyrimidine dimers in DNA
they distort the DNA structure and result in errors during DNA replication
branch migration
process that determines the length of heteroduplex DNA on chromatids
what process does not occur during recombination?
DNA polymerization
what is not a characteristic of IS elements
copy of IS becomes integrated at a new location
what trinucleotide repeat disorder isn’t consistent with hairpin formation?
Friedreich ataxia (GAA)
if non-trinucleotdie repeat expansions were present in the coding region of a gene, which mutation is the most likely?
frameshift
which type of mutagen has a structure similar to one of the DNA nucleotides and therefore can work its way into DNA, where is pairs with a nucleotide?
nucleotide base analog
which of the following is a consequence of inactivation of both p53 alleles in a cell?
reduction of p21 synthesis
can p53 bind to DNA lesions?
no
true or false: Alu elements are SINEs that typically encode an important human protein: restriction endonuclease Alu I
false
is P- cis or trans acting?
cis
what technique can be used to identify binding location of Lac repressor protein relative to location of RNA polymerase binding in the regulatory region of the lac operon?
DNase I footprint protection analysis
how does the phage know if bacteria are actively growing?
protein cII is degraded by proteases
binding cII/cIII to PL triggers the expression of what leading to the lysogenic cycle?
integrase
if the CAP binding region is mutated, what effect would you expect to observe?
the lac operon will exhibit a basal level of transcription
which yields the least amount of transcription of the trp operon
trpP-/tryptophan absent
which yields the most amount of transcription of the trp operon
trp+/tryptophan absent
