Introduction to Veterinary Histology

Question 1

This is a modified bright-field microscope. The usual condenser is replaced by one that causes light to strike the image oblique angle.

Answer 1

dark-field microscope

Question 2

This is visible light microscope uses an ultraviolet light source

Answer 2

Flourescence microscope

Question 3

This type of microscope uses an ultraviolet radiation source emitting wavelengths between 1000 to 3000 angstrom.

Answer 3

Ultraviolet microscope

Question 4

This is used in histology has a resolution limit of about 2200. Stained sections are necessary for maximal effectiveness of this type of microscope because of living tissue transparency.

Answer 4

bright-field microscope

Question 5

are useful adjuncts when tissue architecture is a minimal concern

Answer 5

cytological studies

Question 6

a concern for the altered morphology and function resulting from fixation and subsequent processing

Answer 6

freezing technique

Question 7

Frozen section such as intra-operative biopsy samples are processes rapidly for examination.

Answer 7

freezing technique

Question 8

one in which fresh biopsy samples are frozen to liquid nitrogen temperatures (-1700C) while under vacuum

Answer 8

freeze drying technique

Question 9

is usually applied to studies conducted with light microscopy.

Answer 9

histochemistry

Question 10

applicable to electron microscopy

Answer 10

cytochemistry

Question 11

useful for histological studies of dynamic cellular processes, tissue sections are incubated with radioactively labeled substances

Answer 11

autoradiography

Question 12

useful with light and electron microscopic investigation for the identification and localization of potentially antigenic substances. The purified extract of the desired substance is prepared and injected as antigen into another another animal, the animal develops an immune response and produces antibodies, antibodies are isolated and purified

Answer 12

immunocytochemistry

Question 13

A fluorescent dye, usually fluorescein isothiocyanate (FITC), is complexed with the antibody

Answer 13

direct fluorescent antibody technique

Question 14

Preparation techniques for this microscopy are similar to those used fro transmission electron microscopy, but sectioning and staining are not required.

Answer 14

scanning electron microscopy

Question 15

encompasses all those structural features studied by direct visual inspection, palpation, and dissection

Answer 15

gross anatomy

Question 16

the study of structures which includes minute parts that can not be seen by naked eye

Answer 16

microscopic anatomy

Question 17

study of organ structure and function, commonly studied with a microscope

Answer 17

organology

Question 18

emphasizes structural and functional organ differences and/or similarities between and among various animal groups

Answer 18

comparative organology

Question 19

uses the transmission and scanning electron microscopy. Insights of inorganic chemistry, organic chemistry, biochemistry apply to cells and tissues

Answer 19

ultrastructural cytology

Question 20

This is the study of the microanatomy of cells tissues and organs as seen through the microscope. It examines the correlations between structure and function.

Answer 20

histology

Question 21

father of histology

Answer 21

Marie F.X. Bichat

Question 22

tissue comes from French word “tissu” which means

Answer 22

texture or weave

Question 23

derived from the Greek “histos” (web) and “logos” (study) means the study of tissues

Answer 23

histology

Question 24

building blocks upon which the organs are constructed in logical orderly, and consistent ways

Answer 24

tissues

Question 25

Basic group of tissues (5)

Answer 25

1. Epithelial tissue
2. Connective tissue
3. Muscular tissue
4. Nervous tissue
5. Adipose tissue

Question 26

group of similar cells and their extracellular products performing specific functions or
related functions

Answer 26

tissue

Question 27

permits direct observation of living cells

Answer 27

cell culture

Question 28

most common method used in preparing specimens for histology, diagnostic, histopathology, morphological research with light microscopy

Answer 28

paraffin technique

Question 29

chemical fixation

Answer 29

used on histological specimens for the primary purpose of stopping postmortem autolysis

Question 30

examples of chemical fixatives

Answer 30

formaldehyde, glutaraldehyde, paraformaldehyde, ethyl alcohol, acetic acid, mercuric chloride, picric acid, potassium dichromate, chromic acid, osmic acid

Question 31

induce changes in cells similar to those occurring when heat is applied to an egg.

Answer 31

coagulative fixation

Question 32

this react with cellular biochemical components, they do not induce the marked morphological changes of coagulative fixatives

Answer 32

additive fixative

Question 33

actions of most fixatives (6)

Answer 33

1. Prevent postmortem autolysis by inactivation hydrolytic enzymes
2. Facilitate sectioning of tissues by hardening them
3. Enhance staining by acting as mordant
4. Minimize the leaching of many constituents resulting from subsequent processing
5. Stabilize structural components as possible
6. Protect the histologist through their antiseptic properties

Question 34

dehydrating agents

Answer 34

1. Ethanol
2. Butanol
3. Dioxane
4. Isopropanol