Introduction to Microbiology Flashcards by Rachel Dee | Brainscape

Q

Where can infection be found?

A

Essentially everywhere including:
dental caries and periodontal disease
infectious disease
intensive care
haemotology and oncology patients
children
general practice
hospital acquired infections (HAI)

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Q

What are 5 possible infecting agents?

A

bacteria
viruses
fungi
parasites
prions

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Q

What specimen types are sterile sites?

A

blood
CSF
lung
bladder

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Q

What specimen types are non-sterile sites?

A

skin
nasopharynx
urethra
gut

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Q

Specimen collection for bacterial culture:

UTI

A

mid-stream urine (MSU)

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Q

Specimen collection for bacterial culture-:

chest infection

A

sputum:

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Q

Specimen collection for bacterial culture:

tonsillitis/pharyngitis

A

throat swab

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Q

Specimen collection for bacterial culture:

wound or site of infection

A

swab or pus (swabs are sent in bacterial transport medium)

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Q

Specimen collection for bacterial culture:

diarrhoea

A

faeces

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Q

Specimen collection for bacterial culture:

bacteraemia

A

blood culture

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Q

Specimen collection for bacterial culture:

meningitis

A

cerebro-spinal fluid (CSF)

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Q

What aren’t visible under light microscope?

A

viruses

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Q

What is unstained microscopy used to see?

A

Pus cells (e.g. in urine, CSF)

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Q

What is stained microscopy used to see?

A

Parasites ( e.g in faeces)

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Q

What is a gram stain used to see?

A

bacteria and yeasts/fungi

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Q

What is a ZN stain or auramine stain used to see?

A

mycrbacteria

Q

Describe gram staining and microscopy.

A

rapid= telephone urgent information
not sensitive
can’t usually identify particular species

Q

Describe bacterial culture.

A

slow
sensitive
culture conditions suitable for the expected species