Immunology Flashcards
What are the 2 main components of the immune system?
- Innate Immunity
2. Adaptive Immunity
What is the innate immune system and name several effector cells?
Available for immediate defense with no prior exposure required!
Components: Physical and chemical barriers, Circulating cells, complement, cytokines, interferons, defensins
Cells: Neutrophils, eosinophils, mast cells, macrophages, dendritic cells
What components of complement lead to more inflammation?
Anaphylatoxins = C3a and C5a
What is the final outcome of complement activation?
Stimulates activation of terminal complement components (C5b-C9-membrane attack complex)
What is a TLR?
Toll-like receptor - recognizes intracellular and extraceullar bacteria/virus
On neutrophils - Result in cytokine production = Inflammatory response
TLR2 - Bacterial lipopeptide
TLR4 - LPS
What is a NLR?
NOD-like receptor - recognizes intracellular bacteria/virus
On neutrophils - Result in cytokine production = Inflammatory response
NLRs = Bacterial lipopeptides
What are cytokines and chemokines?
Cytokine: Proteins that are made by affector cells to affect behavior of other cells
Chemokines: Cytokines that are important for chemotaxis
Name the 3 cytokines important for inflammation/fever.
IL-1
IL-6
TNFa
What are 2 major chemokines?
IL-8
CXCL1
What are the 3 major cell types of adaptive immunity?
Lymphocytes (B and T cell)
Antigen presenting cells
What is humoral immunity?
B cells recognize antigen through surface bound Ig> Once activated to become a plasma cell or long-lived memory cell = Secrete Ig, which bind pathgens in extracellular space - leading to their destruction through phagocytosis and complement binding
What is cell mediated immunity?
T cells (via T cell receptors) recognize INTRACELLULAR antignes on cell surface of antigen presenting cells (DO NOT bind free antigen, unlikely B cells)! Based on specialized cellular gylocproteins (gene cluster) = Major Histocompatibility Complex (MHC). Once activated, T cell differentiate into cytotoxic cells (CD8+) or helper cells (CD4+)
Cytotoxic T cells are CD___+.
CD8+
Helper T cells are CD___+.
CD4+
Name 3 antigen presenting cells.
Macrophages, dendritic cells, B cells
What is the role of an antigen presenting cell?
Along with MHC internalize, process, and present antigen+MHC on their cell surface to be recognized by T cells
Where do B cells and T cells “live” in the body?
B cells = BM
T cells = Thymus
What is colonal selection?
Single lymphocyte progenitor produces millions of cells with DIFFERENT and SPECIFIC antigen receptor on each cells - occurs through rearrangement of gene segments in variable region of antigen receptors EACH LYMPHOCTE EXPRESSES ONLY 1 SPECIFIC RECEPTOR
What happens once a lymphocyte is activated?
Interaction of the receptor with a foreign antigen with sufficient binding = activates lymphocyte → Produces CLONES with the SAME SPECIFIC receptor. Clones are known as effector cells, capable of eliminating antigens
How do T cells differ from B cells in term of differentiation?
T cells are MHC restricted!! T cells can recognize foreign antigen only in form of peptide bound to a self MHC molecule on APC.
What is positive selection?
T cells must be able to recognize the body’s own MHC molecules (since they are MHC restricted and can only recognize a foreign antigen when it is bound to a self MHC molecule on an APC)
What is negative selection?
T cells must be able to recognize self peptides bound to self MHC molecules and become self tolerant. Cells binding with high affinity are deleted = negative selection
What happens when a T cell fails positive and negative selection?
It undergoes apoptosis
Estimated that 98% of immature T cells die this way
Which lymphocyte is produced throughout life?
B cells
What are the major steps in lymphocyte migration to sites of inflammation?
- Teter and rolling: Based on P and E selectins on endothelial cells or L-selectin (LN). Rolling mediated by binding of integrins (WBCs) to VCAMS or ICAMS (on endothelial cells)
- Activation: Baed on chemokines (IL-8)
- Arrest: Based on b integrins to ICAMs asn MadCAMs
What portion of an antibody binds to the antigen?
Variable region (Fab) - varies extensively to match antigen variety
What portion of an antibody binds to the APC?
Constant region (Fc) - leads to recruitment of additional immune cells and destruction of pathogen
What determines the antibody isotype and name the 5 types of antibodies?
Constant (Fc) region determines the isotype
IgM (pentamer), IgG, IgD, IgE, IgA (Dimer)
How is a B cell activated?
Antigen binding to transmembrane B Cell antigen receptor stimulates clonal expansion (lots of phosphorylations) and differentiation to an antibody secreting plasma cell (short lived) or memory cells (long-lived) occurs
What is a common leukocyte antigen?
CD45 (present on all WBCs) - Transmembrane surface glycoprotein
What is the main function of the MHC?
To process and present pathogenic peptides to 2 distinct classes of T cells
What is the difference btwn MCH I and MCH II molecules?
MHC I: a chain and b2 microglobulin - Does NOT cross the membrane = Expressed on majority of nucleated cells
MCH II: a and b chains - crosses the membrane = Mainly expressed in APCs and in thymus (self recognition)
Great polymorphorism is present in MCH molecules
What is DLA?
Dog Leukocyte Antigen
What is the role of MCH Class I in antigen processing and presentation?
Presents peptides to CD8+ (cytotoxic, killer cells)
Pathogens that are found in cytosol (viruses, tumor antigen, some bacteria) = Cell death
Cytosolic pathgenic proteins are degraded by proteosomes and delivered to ER where MHC I molecule is ready , peptide binding occurs = MHC I: Peptide complex transported to surface where it is exposed to CD8+ T cells
What is the role of MCH Class II in antigen processing and presentation?
Present peptides to CD4+ (T helper 1 or 2). Th1 - Acitvate inflammatory cells to kill intravesicular pathogens, Th2 - activate B cells to secrete Ig to eliminate extracellular bacteria/toxin
Antigen exists or is transported to intracellular vesicle and degraded into peptides, which fuse with MCH II and move to cell surface and exposed to CD4+ cells
What is the difference btwn Th1 and Th2?
Th1 = Activate inflammatory cells to kill pathogen (intravesicular) Th2 = Activate B cells to produce Ig (extracellular)
What are the components of the T cell receptor complex?
Resembles the Fab portion of Ig (contains constant and variable region in a and b chains
Assoicated with CD3 complex (intracellular signaling) and CD45 (on all WBCs)
Co-receptor: CD4 or CD8 (some carry both)
What is the superantigen?
Binds ACROSS the MCH:T cell receptor (without processing) = Activation leads to massive production of cytokines by CD4 cells that leads to SIRS!!!
Bacteria (Stap enterotoxins, and some viral proteins)
Discuss the activation of naïve T Cells to effector T cells.
- Naïve T cells migrate to peripheral lymph organs were they are exposed to APCs (MP, dendritic cells, B cells) - Encounter of T cells with an antigen (on APC surface) = Primary immune response
- Recognition of MCH: Antigen complex
- CO-STIMULATORY SIGNAL delivered by same APC - binding of cell surface molecule B7 found on APC to CD 28 on T cell
- Activated T cells makes IL-2 = Drives T cell growth and clonal proliferation = Armed effector cell that is capable of carrying out cytotoxic or helper functions WITHOUT co-stimulation
What 2 steps are required for T cell activation?
- Recognition of MCH: Antigen complex
- CO-STIMULATORY signal delivered by SAME APC (Binding of cell surface molecule B7 found on APC to CD 28 found on T cell)