Histology of CT & ET

Question 1

Light microscope vs electron microscope

Answer 1

Light microscope: 1 micrometer (10^-6 meter) resolution with colored images

Electron microscope: 1nm (10^-9) resolution with non-colored images

Question 2

Types of electron microscopes

Answer 2

Scanning: 3D Images, cell surface features only

Transmission: 2D images, intracellular features visible

Question 3

Slide preparation for Light microscopy:

Answer 3

1. Obtain specimen (biopsy)
2. Preserve specimen (freeze or use formaldehyde)
3. Harden specimen (fix in wax or plastic)
4. Section specimen into sections (1-10 micrometers thick)
5. Place specimen on a slide
6. Stain the specimen

Question 4

Hematoxylin and Eosin stain

Answer 4

Hematoxylin: blue stain targeting acid-based structures like DNA in Nucleus.

Eosin: Pink stain targeting alkaline structures like Proteins in cytoplasm

Question 5

Periodic Acid Schiff

Answer 5

Used for goblet cells

Stains mucus

Question 6

Fluorescent Stain

Answer 6

Stains targeted structures using specific antibodies with fluorescent dye attached to antibodies

Question 7

Answer 7

Simple Squamous ET

Question 8

Answer 8

Simple Cuboidal ET

Question 9

Answer 9

Simple Columnar ET

Question 10

Answer 10

Goblet Cells

Question 11

Answer 11

Non-Keratinized Stratified Squamous ET

Question 12

Answer 12

Keratinized Stratified Squamous ET

Question 13

Answer 13

Stratified Cuboidal ET

Question 14

Answer 14

Non-Ciliated Pseudostratified Columnar ET

Question 15

Answer 15

Ciliated Pseudostratified Columnar ET

Question 16

Answer 16

Areolar CT

Question 17

Answer 17

Adipose CT

Question 18

Answer 18

Reticular CT

Question 19

Answer 19

Dense Irregular CT

Question 20

Answer 20

Dense Regular CT