Fungi, Parasite Flashcards
For fungi,
What is the cell wall made of
What is the cell membrane made of
Does it has a cytoplasm and nucleus?
Glucan, protein, Chitin
Phospholipid, ergosterol
Yes
What are the 3 classifications of fungi
Yeast, Moulds, Dimorphic fungi
For yeast
What’s the size
Is it unicellular/ multicellular
How does it divide
Name 2 example
5 micrometer in diameter
Unicellular
By budding
Candida albicans
Cryptococcus neoformans
For mould
What is the
Unicellular/ Multicellular
Size?
Does it form globular/ filament? What is the product formed
How does it reproduce
Name 2 subdivisions
Multicellular
2-4 micrometer wide
Filament. Hyphae–> Mycelium
By spore, via sexual/assexual reproduction
Aspergillus fumigatus
Dermatophytes
‐ Trichophyton, Epidermophyton, Microsporum
For dimorphic fungus
What determine the form
Name the forms and the criteria
Example?
Temperature
Yeast: 37 degree Celsius
Mould: 25 degree Celsius
Penicillium marneffei, aka Talaromyces marneffei
Name 3 global fungi
Name 1 local fungi
Neutropenia is the risk factor for what
HIV is the risk factor for what?
Candida, Dermatophytes, Aspergillus
T. marneffei
Aspergillus
C. neoformans, T. marneffei
What fungi causes infection/problem in immune competent host?
uncommon, but some can be allergic to the spores
What fungi causes infection in immunecompromised host?
Systemic candidiasis (ie by Candidas)
Cryptococcus neoformans
Aspergillus fumigatus
Talaromyctes Marneffei
What are the 4 classes of antifungal drug
What is their mechanism
Polyenes: complex with ergosterol to disrupt the fungal plasma membrane
Azole: inhibit ergosterol synthesis
Nucleoside analogues (as DNA polymerase inhibitor, reduce DNA synthesis and viral replication)
Echinocandins: Cell wall synthesis inhibitors
Name an example for
Polyenes
Azoles
Nucleoside analogues
Echinocandins
Nystatin, amphotericin B
Fluconazole, itraconazole, voriconazole,
posaconazole
5-flucytosine
Caspofungin, micafungin, anidulafungin
What stain do we use to visualize
Mould
Candidas
Cryptococcus neoformans
What is the method to visual ALL fungi’
What is the culture used
KOH
Gram Stain
Indian ink stain
Biospy (ie obtain sample)
Histopathology (ie observe sample)
Agar and broth culture
Name the component of cell walll that is detected in antigen testing to detect virus
What is the antigen we use for C.neoformans
What is the antigen we use for Aspergillus
Name another method to detect Aspergillus
What do we look for Patient’s response
Name a fungi that can use antibody detection
Beta-D-glucan
Capsular polysaccharide
galactomannan
Nucleic acid detection
Antibody detection
Biospy and histopathology
T. marneffei
Is Candida albican
A yeast/mould/dimorphic fungi
Name 3 important species
What is the host defense against it
Yeast
-C. parapsilosis
-C. tropicalis
-C. krusei
-Intact skin
-Neutrophils, monocytes
For Candidas albican
What is the reservior
Is it local/global?
What are the 8 risk factors for that
Skin, GI tract, female genital tract
Global
-Broad spectrum antibiotics:
selective pressure
-Hospitalization
-ICU
-Intravenous lines
-HIV (suppressed Immune system)
-Neutropenia (Low neutrophll count)
-Transplant (use of immune suppressor)
-Steroid (same as above)
What can Candidas albican cause
In immune competent host
In immunocompromised host
-Vaginal thrush
Refractory superficial infections
-Oral thrush
-Mucocutaneous candidiasis
Systemic candidiasis
-Candidemia: one of the major causes of nosocomial blood
stream infection
-Disseminated infection: Eye, skin, kidney
-Hepatosplenic candidiasis
-Any organ
How do we diagnosize the Candida albicans
By visualization?
By culture
By Antigen detection?
With gram stain
-Agar plates e.g. pus
-Broth culture e.g. blood
-Germ tube formation in serum, biochemical tests
Serum: -D-glucan
How do we treat
Superficial candidiasis
Systematic candidiasis?
Topical treatment (e.g. nystatin)
-Systemic treatment in refractory cases (e.g. fluconazole)
-Systemic treatment
-Fluconazole, amphotericin B
Why shld we prevent Candida albicans by avoid antibiotics overuse
What drug do we use to prevent? In what situation?
It prevents overgrowth of fungi due to disrupted flora and microbiome
Fluconazole prophylaxis for transplant recipients
Can we avoid exposure and do vaccination
No
What is the defintion of Parasitism
any reciprocal association in which a species depends upon another for its existence.
What is the defintion of a parasite
the species that derives all the benefit from the association.
What is the host
the harbouring species
What is the word infection and infestation in parastie
Infection: Invasion of endoparasites
Infestation: External parasitism by ectoparasites
How can we classifiy parasite according to their
Dependence on host
Temporal relationship with host
Physical relationship with host
obligatory (complete dependence) or facultative
temporary or permanent
ectoparasite or endoparasite
How can we diagnosize parasitic infecion by demostrating parasitic in appropriate specimen?
If we detect antigen/antibody in blood/other body fluid, what is it called
What parasites will we culture and is it common?
If we wanna dianosis via nucleic acid, what have to be done
direct microscopic examination, staining, or tissue sections
Serology
Protozoa
PCR