Final Labs Flashcards
How to process fresh semen
Collect
Evaluate
Mix with extender that contains antibiotic
Inseminate mare within 2-4 hours after collection
How to process extended/cooled semen
Test semen with various extenders before the semen
Collect and evaluate
Mix with extender (ratio of semen to extender 1:4)
Place in cooling container
Inseminate mare within 48 hours after collection
How to process frozen semen
Test semen in various freezing extender Collect and evaluate Mix with freezing extender Package semen in straws Freeze semen and store in liquid nitrogen Thaw semen and use when needed
What should you do to grossly evaluate semen
Volume
Appearance
PH
Who gives the highest volume of semen
Boar
How can you determine concentration of semen
Spectrophotometer
Hemocytometer
Nucleocounter
What is the gold standard to evaluate semen morphology
Phase contrast microscopy
What are two common stains for semen
Eosin nigrosin (negative staining) Diff quick
What does the “supravital” stain show
Alive sperm- intact membranes, white
Dead sperm- damaged membranes, red
Where do primary sperm defects originate
Seiminiferous epithelium
Where do secondary sperm defects originate
Epididymis, deferent duct, urethra
Where do tertiary sperm abnormalities originate
Human errors
Does a primary sperm defect mean it is a major sperm defect?
NO
What is the most common cause of infertility in canine and feline
Breeding at the wrong time
What cells will you see on vaginal cytology during proestrus
Parabasal to intermediate
What cells will you see on vaginal cytology during estrus
Intermediate to superficial
What cells will you see on vaginal cytology during diestrus
PMNs
What cells will you see on vaginal cytology during anestrus
Basal cells
What does P4 estimate
LH peak and ovulation