FECT procedure Flashcards
- With an applicator stick add_______ of feces to_____ of formalin in a centrifuge tube and stir to form a suspension
- Strain the suspension through the_____ mesh sieve or____ layers of wet surgical gauze directly into a different centrifuge tube or into a small beaker. Discard the gauze
1.0 - 1.5 g
10 mL
400 um
2
- Add more_______ to the suspension in the tube to bring the total volume to 10 mL
- Add______ of ether (or ethyl acetate or gasoline) to the suspension in the tube and mix well by putting a rubber stopper in the tube and shaking vigorously for____ seconds.
10% formalin
3.0 mL
10sec
- Remove the stopper and place the tube in the centrifuge; balance the tubes and centrifuge at 400-500g for______
- Remove the tube from the centrifuge; the contents consist of_____ layers: (a) top layer of ether (or ethyl acetate or gasoline), (b) a plug of fatty debris that is adherent to the wall of the tube, (c) a layer of formalin, and (d) sediment
2-3 minutes
four layers
- Gently loosen the plug of debris with an applicator stick by a spiral movement and pour off the top____layers in a single movement, allowing the tube to drain inverted for at least five seconds. When done properly a small amount of residual fluid from the walls of the tube will flow back onto the sediment
- Mix the fluid with the sediment: (sometimes it is necessary to add a drop of saline to have sufficient fluid to suspend the sediment) with a disposable glass pipette. Transfer a drop of the suspension to a slide for examination under a coverslip; an iodine-stained preparation can also me made
3 layers
- Examine the preparations with the____ objective, or if needed for identification, higher power objectives of the microscope in a systematic manner so that the entire coverslip area is observed. When organisms or suspicious objects are seen, switch to higher magnification to see more detailed morphology of the object in question.
10x
_______+______
Mix and emulsify
Strain into the beaker
Remove and throw the gauze
Transfer back the suspension to the beaker
Centrifuge for____ mins
Remove by decanting the superatant, break the sediment
Add____ of Nss, mix
Add____ Ethyl Acctate Mix gently
centrituage____ mins
Observe the layers
Decant, observe
1g of stool + 10mL Formalin
10mins
7ml
3ml
3
Zinc sulfate concentration
33%
Zinc sulfate SG
1.18-1.20
Sheather’s sugar floatation technique
SG
1.27
Acid Ether Concentration Technique
HCl concentration
40% HCI
Unsa tung ginagamit ug homocytometer?
McMaster technique
Unsa ginagamit sa Brine Floatation
Table salt
Unsa ginagamit sa Sheather’s sugar floatation?
Table sugar
Purpose of 40% HCl
Dissolve albuminous material
FECT
is not recommended for eggs of
and larvae of
Fasciola spp.
Strongyloides stercoralis.
