Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Parasitology LAB > FECT > Flashcards

FECT Flashcards

1
Q

ADVANTAGES of CONCENTRATION TECHNIQUE

A

Maximizes the number of organisms detected which may be too scanty to be seen by direct microscopy alone.

Separate parasites from fecal debris

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

DISADVANTAGES of CONCENTRATION TECHNIQUE

A

Destroys trophozoite stages

Distorts any cellular components

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

ADVANTAGES of SEDIMENTATION TECHNIQUE

A

Recovers most ova, cysts, and larvae.

Morphology of most parasites is retained.

Less risk of infection from bacteria and viruses.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

DISADVANTAGES of SEDIMENTATION TECHNIQUE

A

Preparation contains debris.

Some parasites do not concentrate well.

Ether is flammable and formalin is an irritant.

Liquid feces do not concentrate well.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

ADVANTAGES of FLOATATION TECHNIQUE

A

The concentrate is clear of debris

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

DISADVANTAGES of FLOATATION TECHNIQUE

A

Delay in examination can result in distortion

Larvae and some fluke eggs do not concentrate

Frequent checking of specific gravity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

2 Sedimentation techniques

A

Acid Ether Concentration Technique + 40% HCI

Formalin Ether/Ethyl Acetate Concentration Technique

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

4 Concentration techniques

A

Brine Floatation (NSs)

Zinc Sulfate Floatation

Sheather’s Sugar Floatation

McMaster Technique

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

A procedure that allow for the detection of parasitic elements (eggs, larvae, oocysts and cysts) that may be missed when examining only a direct wet preparation

A

FECT

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

FECT

A procedure that allow for the detection of parasitic elements (ELOC) that may be missed when examining only a direct wet preparation

A

eggs
larvae
oocysts
cysts

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

This technique is not recommended for eggs of_______ and larvae of______

A

Fasciola spp.

Strongyloides stercoralis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

FECT

Examples of parasitic stages that might be seen under the microscope

A

Faciola egg
Entamoeba cysts.
Ancylostoma Egg.

Bacterial rods
Isospora oocyst
Isospora canis (coccidia) oocysts
Coccidia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

_________ in General

Are techniques used to separate parasites from fecal debris and increase the chances of detecting parasitic organisms when these are in small numbers.

A

Concentration Techniques

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

2 types of conc. techniques

A

Sedimentation technique
Floatation/Flotation technique

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Floatation techniques (3)

A

Saturated saline
Zinc sulfate
Sheather’s sugar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Sedimentation techniques

A

Saline
Formol ether

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Use solutions of lower specific gravity than the parasitic organisms, thus concentrating the latter in the sediment.

A

Sedimentation technique

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

are recommended for general diagnostic laboratories because they are easier to perform and less prone to technical errors.

A

Sedimentation techniques

19
Q

_____ is the sedimentation technique used at CDC of USA and is recommended by the WHO

A

FECT

20
Q

Floatation Techniques

Most frequently used:

A

zinc sulfate

Sheather’s sugar

21
Q

Floatation techniques

Use solutions which have _________ sg than the organisms to be floated so that the organisms rise to the top and the debris sinks to the bottom together with the solution, since mas mabigat sila essentially.

A

higher specific gravity

22
Q

The main advantage of this technique is to produce a cleaner material than the sedimentation technique.

A

Floatation technique

23
Q

The disadvantages of most floatation techniques are that the walls of____ and ____ will often collapse, thus hindering identification.

Also, some parasite eggs do not float

A

eggs and cysts

24
Q

is a widely used sedimentation technique for the diagnosis of intestinal protozoa in preserved stool samples

A

The formalin-ether concentration technique (FECT)

25
Q

The most commonly used fixatives for stool preservation are either ______ or _______

A

formalin

sodium acetate-acetic acid-formalin (SAF)

26
Q

The use of________ instead of_____ avoids the flammability problem of ____, and EA can be used with specimens preserved in formalin, MIF or SAF.

A

ethyl acetate

ether

27
Q

can be used with specimens preserved in formalin, MIF or SAF.

A

Ethyl acetate

28
Q

FECT lacks sensitivity for the diagnosis of________

A

helminths

29
Q

Its diagnostic accuracy is inferior to that of the widely used Kato-Katz technique for the diagnosis of:

A

Schistosoma mansoni
Common soil-transmitted helminths
(A. lumbricoides, T. trichiura, and hookworm)

30
Q

______ conical centrifuge tube

A

15 mL

31
Q

4 layers after final centrifugation

A

Top layer of ethyl acetate/ether/

A plug of fatty debris that is adherent to the wall of the tube

A layer of formalin
The sediment ( which should contain the parasite in search of)

32
Q

What can we detect using Sedimentation

A

•Heavy eggs (Ascaris egg)
• Operculated eggs (Trematodes)
• Larvae (Strong stercolaris.)
• Cysts

33
Q

What can we detect using Floatation

A

• Non Operculated eggs
Trematodes (Schistosoma.)
Cestode
Nematode (Hookworms)
• Cysts

34
Q

Less distortion of cysts occurs with this technique than with zinc sulfate method and it is more effective in concentrating formalinized specimens.

A

FECT

35
Q

______ eggs may be missed, however, and concentration of_____ cysts and____ cysts may not be very good.

A

Hymenolepis nana

Giardia lamblia

iodamoeba butschlii

36
Q

Ether, ethyl acetate or, if these solvents are unavailable,________.

A

gasoline

37
Q

is highly volatile and will ignite and explode quickly if there is an open flame or spark nearby.

A

Ether

38
Q

FECT

This procedure makes use of 10% formalin which is an_______, and ether, which can______ in the stool.

A

all purpose fixative

dissolve neutral fats

39
Q

FECT

This is useful in the recovery of both

A

helminth eggs

protozoan cysts

40
Q

FECT can also be done with______ stools

More parasites can be recovered from formalin preserved samples.

Parasite morphology is also better preserved in formalin than in PVA.

Sediments from FECT can be stored for a long period of time.

A

formalin-preserved and PVA-preserved stools.

41
Q

Those who have tried ethyl acetate claim that it is more efficient than ether in the recovery of______ and _____

However, ethyl acetate is not as efficient as ether in the extraction of____ or _____ from the stool.

A

cestode eggs and Giardia cysts

fat or mucoidal material

42
Q

Formalin is an all purpose fixative.

A_____ concentration is recommended for protozoan cysts, while a ____concentration is recommended for helminth eggs and larvae.

A

5%

10%

43
Q

Formalin solution may be buffered with_______ to preserve the morphological characteristics of the organisms.

Preserved stool can be concentrated using___________

A

sodium phosphate

formalin-ether/ ethyl acetate concentration technique (FECT)

Parasitology LAB (37 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions