Exam 2 Microbial Metabolism PT 1

Question 1

Role of ATP as an intermediate between catabolism and anabolism

Answer 1

Catabolism breaks down molecules and produces ATP
Anabolism uses ATP to combine molecules

Question 2

Activation Energy

Answer 2

Amount of energy you need to make something happen

Question 3

Reaction Rate

Answer 3

How many collisions occur
Increases by temperature rising and pressure
Enzymes speed up chemical reactions

Question 4

Components of an Enzyme

Answer 4

1.) Apoenzyme: protein part
2.) Cofactor: non-proteins
- Inorganic: Ions
- Organic: Coenzymes
3.) Holoenzyme: Full functioning unit

Question 5

Substrate

Answer 5

a molecule that an enzyme reacts with
- Binds to active sites on emzymes

Question 6

Mechanism of enzymatic action

Answer 6

1.) Binds with active site on enzyme
2.) Substrate fits on site
3.) Cell finds a new AB
4.) AB are released from active site
5.) AB bond is broken and is now free to bind to another substrate

Question 7

Factors that can influence enzymatic activity

Answer 7

Substrate Concentration- no substrate means the enzyme does nothing
- Max velocity- enzyme goes as fast as it can

Temperature: will increase the rate which will denature the enzyme and then the enzyme is non-functional
pH- The more basic, the more enzymic activity

Competitive inhibitors: binds to active site till it cant bind anymore.
- Changes active site shape and then it cant act on substrate
Feedback Inhibition: end-product pathway inhibits enzyme at the beginning