Controlling Microbial Growth in Environment pt1 Flashcards
Disinfectant
destruction vegetative pathogens on inanimate object
Antisepsis
Destruction vegetative pathogens on living tissue
Sterilization
destruction/removal of all microbial life, including endospores (not prions)
bacteriocidal
kills bacteria
bacteriostatic
inhibits bacterial growth
Major actions of antimicrobial agents
cell membrane- leakage
proteins- loss of function
nucleic acid- mutations
Major actions of antimicrobial agents
Cell Membrane
Leakage
Heat, hypertonic solutions, phenolics, alcohols
Major actions of antimicrobial agents
Proteins
Loss of function
Heat, phenolics, halogens, alcohols, aldehydes, ethylene oxide
Major actions of antimicrobial agents
Nucleic Acid
Mutations
Ionizing/non-ionizing radiation, aldehydes, ethylene oxide
Factors to consider when selecting a method of microbial growth on environment
Nature Site Treatment
Environmental conditions
Susceptibility of microbe involved
Nature Site Treatment
humans, medical instruments, heat sensative material
Environmental conditions
Temperature and pH, presence organic matter
Susceptibility of microbe involved
prions, endospores, enveloped v.s. non-enveloped viruses
Modes of action, and uses, of physical methods of controlling microbial growth in environment
Heat- damages proteins and membrane
Moist heat sterilization- boiling @100c, autoclaving
Dry heat sterilizations- direct flaming and hot air sterilization
Pasturization
How filtration controls microbial growth in environment
liquid/gas through filter with pore small enough to trap microbes
Filter is sterile. Microbes still alive
