Enzymes Flashcards
Digest?
To break down
Synthesis
Build large molecules with small molecules
Monomer
One molecule
Polymer
Repeating number of similar small molecules
Catalyst
Speeds up chemical reactions
Substrate
The beginning reactants
Product
The end result
Enzyme
A biological Catalyst, acidic and strong
What does benedict test for
reducing sugars
What does biuret test for
protein
What does iodine test for
starch
What does emulsion test for
lipids/fat
What colours to benedict turn in a reaction
P=Red, N=Blue
What colours to Biuret turn in a reaction
P=Purple N=Blue
What colours to iodine turn in a reaction
P=Blue/Black N=Brown
What colours to ethanol turn in a reaction
P=Cloudy N=Clear
What is The lock and Key model
A model that represents a substrate fitting into an active site
What does De-natured mean
It is when a part of a cell, changes shape because of a change in pH or temperature
3 examples of main digestive enzymes
Lipase, Protease, Amylase
Core practical: pH and enzyme
One tube of starch
One tube of Amylase
Warm up
Mix tubes and start timer
every 30, remove small sample and test with iodine
Stop when change stops
How to test for Sugars
Add Benedict’s reagent to the food and boil in a water bath.
How to test for starch
Add iodine reagent to the food.
How to test for protein
Add Biuret reagent to the food.
How to test for fats
Add ethanol to the food to dissolve the fat then add water.
Why is amylase not produced in the stomach
Too high pH which would cause the denaturing of the active site
Explain why catalysis by enzymes is important for life processes
Because reactions happen much faster
