enzymes

Question 1

Test for reducing sugar

Answer 1

Benedict’s test, add benedict’s reagent and heat in the boil water bath for 2-3 minutes. the solution turns brick-red if there is glucose

Question 2

test for protein

Answer 2

add biuret reagent to the solution and gently shake. if the solution turns purple then there is a protein

Question 3

test for starch

Answer 3

add iodine solution to the solution. shake the mixture gently. if there is starch, then the solution turn blue-black

Question 4

test for lipids

Answer 4

ethanol is added to the solution and gently shaken. then it is poured into a test tube that contains an equal volume of distilled water. if there is lipid then the solution turn milky-white

Question 5

test for vitamin C

Answer 5

add dcpip, the solution the colourless. the less the volume of dcpip is used for the colour change, the stronger the vitamin c solution.

Question 6

what are enzymes

Answer 6

a protein that work as a biological catalyst

Question 7

which enzyme break down fats, and its product

Answer 7

lipase, fatty acid and glycerol

Question 8

which enzyme break down starch, and its product

Answer 8

amylase, maltose

Question 9

what is denatured

Answer 9

if the enzyme is in a condition that is too high temperature, the enzyme gets denatured. if it gets denatured, their active site change shape and so that they can operate normally. once they get denatured, they can’t come back to original state

Question 10

why a type of enzyme only work for specific molecules

Answer 10

each enzyme has its own unique active site that the substrate molecules can fit perfectly.

Question 11

______ + enzyme then it becomes
________________
then product is formed

Answer 11

substrate,

enzyme-substrate complex

Question 12

what effects the enzyme activity

Answer 12

temperature, pH, and surface area of the substrate