biotechnology Flashcards
what is biotechnology
using living organisms to carry out processes to make out the substance we want
why do we use microorganisms such as bacteria for genetic engineering and biotechnology
they are easy and quick to reproduce
no ethical issues
same genetic code with all the organism
equation for fermentation
glucose -> ethanol + carbon dioxide
how can we use ethanol
for fuels
what makes the dough rise in bread making
carbon dioxide
what is the organism that involves in fermentation
yeast
what happens to the alcohol that yeast produce during bread making
they get to break down when the bread is baked. during baking, the heat also kills yeast.
what process does the yeast do during fermentation
anaerobic respiration
what is lactase
an enzyme breaks down the sugar found in milk
why do all the human produce lactase when they are a baby
to digest the only source of food
the purpose of making lactase
some people don’t produce lactase when they become an adult so they will get sick if they drink milk or eat cheese
why is there a water jacket around the fermentor
to control the temperature that can affect enzymes’ activity
why is there air supply in the manufacture of penicillin
bubbles provide oxygen and mix the nutrients with penicillium together
what is the action needed for the fermentor of penicillin
stirring
what is genetic engineering
changing genetic material of an organism by removing, changing or inserting individual genes
purpose of fermenter
to grow bacteria or fungi to a large amount
the process of genetic engineering
desirable gene is identified and located
the gene is cut by the restriction enzyme
the plasmid( bacterial cell) is cut open at specific points, by the same restriction enzyme. now it is called the vector
the enzyme ligase add desirable gene into the vector
the vector is re-inserted into the bacterial cell
place in a fermenter to reproduce them into large amount
bacteria is removed
the function of restriction enzyme
cut the DNA into pieces and leaving sticky ends at the end of the pieces
the function of ligase enzyme
combine the DNA pieces