DX Test For Derm 2

Question 1

Fungal culture

Answer 1

Early color change is followed by dermatophyte growth
Dermatophytes: change of color to red within 3-14d
Contaminant fungi: change of color after 10-14d
Examine culture daily for first 10d

Question 2

Fungal ID

Answer 2

Macroscopic exam of colony
Microscopic appearence of the macro - and microconidia

Question 3

Fungal culture ID steps

Answer 3

Tape to colony surface
Place sticky side down w lactophenol cotton blue
Examine at 10-40x magn

Question 4

Skin biopsy

Answer 4

Required diagnosis for:
Neoplasia
Autoimmune skin disease
Dermatomyositis
Sebaceous adenitis
Zinc-responsive dermatosis
Follicular dysplasia

Question 5

Skin biopsy useful

Answer 5

Some parasitic (dermoidicosis)
Bacteria (mycobacteria)
Fungal (deep) skin diseases
Sterile dermatosis
Paraneoplastic syndromes
Ruling out some differential diagnosis

Question 6

When to biopsy

Answer 6

History /clinical exam suggest a dermatosis which requires histo pathological diagnosis
When reasoned diagnostic approach has not produced a diagnosis
Treatment has failed, disease requires a treatment with potential significant side effects
Nodular, ulcerative, vesicular lesions