DNA replication Flashcards
Describe the structure of DNA
double helix
sugar phosphate back bone
4 nitrogenous bases
name the 4 nitrogenous bases
1) Adenine
2) guanine
3) cytosine
4) Thymine
how many hydrogen bonds join Adenine and thymine and what are they?
2
how many hydrogen bonds join cytosine and guanine and what are they?
3
The 2 strands of DNA run in what direction?
antiparallel
which end is a nucleotide added to a growing DNA strand?
3 prime
where does the energy come from to form the phospodiester bond between the nucleotides?
a nucelotide triphosphate - releases 2 phosphates
where does replication start in DNA?
at the origin of replication
what is the function of the enzyme topoisomerase?
corrects overwinding ahead of replication forks
what is the function of helicase?
untwist the DNA at the replication forks
what is the function of primase?
serves as a starting point for DNA synthesis by synthesising a short RNA primer
what is the function of DNA polymerase lll?
catalyses synthesis by pulling the DNA strand through the enzyme
what are the sections of DNA called in the lagging strand?
okazaki fragments
what is the function of DNA polymerase l?
replaces the RNA primer with DNA nucleotides
what joins together the okazaki fragments?
DNA ligase