directed reading Flashcards
how many genes coded for proteins that induce tolerance?
2 out of 4
what is reverse transcription?
converting mRNA into DNA
how do you measure relative mRNA levels?
reverse transcriptase quantitive PCR
what is the benefit of adding a fluorescent probe?
each doubling of DNA doubles the amount of luminescence - small amount of fluorescence doesn’t show up on camera so only shows up when enough DNA has been doubled - can measure between two samples when camera starts to pick up PCR cycle is in exponential phase - measure the difference in amount of RNA
what do nuclear run on assays measure?
rates of mRNA production from the nucleus
what do you label the nuclei with?
biotin
what is Chromatin-immuno-precipitation?
quanitfy binding between specific gene DNA sequences and a nuclear protein
