dna manipulation and pcr

Question 1

ligase

Answer 1

an enzyme that joins molecules together by catalysing the formation of phosphodiester bonds

Question 2

polymerase

Answer 2

an enzyme that synthesises a polymer from monomers, such as forming a dna strand from nucleic acids

Question 3

primer

Answer 3

a short, single strand of nucleic acids that acts as a starting point for polymerase enzymes to attach

Question 4

restriction endonuclease

Answer 4

cut dna into smaller fragments using blunt or sticky ends

Question 5

order of pcr and temps

Answer 5

1. Denaturation, 90-95 degrees
2. Annealing, primers anneal to each dna strand at either side of the target sequence
3. Elongation, taq polymerase extends the primers