DNA Extraction Lab

Question 1

3 basic steps in DNA Extraction

Answer 1

1. cell must be lysed( broken open) to release the nucleus
2. Nucleus is open to release DNA
3. Once the DNA is released it must be precipitated out of the solution

Question 2

reagents

Answer 2

a substance or mixture for use to produce a chemical reaction

Question 3

reagents required

Answer 3

1. salt-
2. detergent-breaks down lipid membrane-
3. alcohol–ethanol precipitates the dna

Question 4

both cell and nuclear membrane are made mostly of what macromolecule

Answer 4

lipids (phospholipid)

Question 5

In order for the cell to be lysed what must first occur

Answer 5

The lipid membrane must first be broken down

Question 6

What reagents are used to break down lipid membranes of cells (. How?

Answer 6

1. Manual grinding
2. detergent solution

Soap molecules mix with fats or lipids, causing structures made if lipids to break apart

Question 7

alcohol–ethanol

Answer 7

precipitates the dna

Question 8

Salt

Answer 8

It provides the Dna with a favorable environment by contributing POSITIVELY CHARGE atoms that neutralizes the normal negative charge of DNA, allowing it to clump together

Question 9

the charge of DNA

Answer 9

NEGATIVE ( also pole/soluble)