DNA extraction Flashcards
Polymerase chain reaction
original strand of DNA
who was PCR discovered by?
Kary Mullis 1983
what are the 4 PCR application?
-used for cloning
-disease diagnosis
-detection of infectious agents
-forensics
archaeology
ancient DNA analysis
Genome sequencing
DNA sequence of the genome of an organism
what happens to the fragments in
You fragment the genome into small fragments
6 applications in genomic sequencing
-disease diagnosis/predication in clinical medicine
-prenatal, new-born screening
-infectious disease epidemiology
-bacterial viral evolution studies
-human population history studies
-personalised medicine
Reverse transcription CPR
synthesis of complementary DNA from RNA. The reverse transcriptase occurs in RNA viruses(retroviruses)
what can be used to study proteins?
polyacrylamide gel electrophoresis(PAGE)
what is PAGE
basic method for analysing cellular protein profiles.
what happens during western blotting(immunoblotting)
Run proteins on a gel and transfer protein of membrane which are detecting specific antibodies to the proteins that you are looking for and then a specific band will be shown.
ELISA- enzyme-linked immunosorbent assays
immunological assay -use to measure antibodies
Proteomics
proteome -complete set of proteins produced by cell, system or organism under set of conditions
what 4 uses can proteomics have
1.protein production
2.stability
3.control and protein modifications
4.protein interactions
