Diagnostic tests Flashcards

1
Q

what is SOAP?

A

S – subjective
O- objective
A – assessment
P - plan

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2
Q

what are the key elements that make up “signalment”

A

characteristics of the animal/group of animals

  • Species
  • Breed
  • Color
  • Distinctive markings
  • Age
  • Sex/reproductive status
  • Use/production status
  • Animal identification
  • Herd information
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3
Q

what is the TPR for average-sized adult dogs and cats?

A

101.0 - 102.5 F

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4
Q

what does the fundic exam allow us to visualize?

A

Visualization of the cornea

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5
Q

What does tonometry measure?

A

Measures intraocular pressure

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6
Q

What does the Schirmer tear test measure?

A

Quantifies tear production

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7
Q

What does the fluorescein stain help detect?

A

for corneal ulcers

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8
Q

Palpable organs in cats & dogs

A
  • Intestines
  • Colon
  • Bladder
  • Kidneys
  • Spleen
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9
Q

Nonpalpable organs in cats & dogs

A
  • Liver
  • Pancreas
  • Stomach
  • Adrenal glands
  • Uterus
  • Ovaries
  • Lymph nodes
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10
Q

Why do we auscult the abdomen of large animals?

A

To listen to abnormal gut sounds, increased gut sounds, or lack of gut sounds

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11
Q

what are the the pros and cons of using diagnostic tests?

A

Pros:
- Aids in diagnosing a problem

Cons:
- Does not replace a thorough history and physical exam
- Tests are expensive
- Some tests can be invasive or dangerous

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12
Q

what parameters are measured on a CBC

A
  • PCV (packed cell volume)/ hematocrit
  • Red blood cell count (RBC)
  • Number of white blood cells (WBC)
  • Hemoglobin
  • Number of platelets
  • Blood film examination
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13
Q

what electrolytes can be measured and know their ionic “charge” (look this up, if you don’t know it).

A

Serum chemistry helps measure electrolytes

  • Glucose (1) & calcium (+2)
  • Proteins
  • Electrolytes
  • Kidney tests
  • Liver tests
  • Muscle enzymes
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14
Q

what proteins can be measured?

A

total protein, albumin, immunoglobin

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15
Q

what liver enzymes that can be measured

A
  • Alkaline phosphatase (ALP)
  • Alanine transferase (ALT)
  • Aspartate transferase (AST)
  • Gamma-glutamyl transferase (GGT)
  • Bilirubin
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16
Q

what are the parameters that can be measured on a urine dipstick

A
  • Blood
  • White blood cells
  • Protein
  • pH
  • ketones
  • bilirubin
17
Q

What can urine sediment detect?

A

crystallization in the urine

18
Q

what are the 4 main types of imaging that is used in veterinary medicine. Know when we might prefer to use one over another?

A
  1. Radiographs (“x-rays”)
    a. Bony tissue
    b. Soft tissue
    c. Without or with contrast
  2. Ultrasound
    a. Soft tissue
  3. MRI (magnetic resonance imaging)
    a. Soft tissue
  4. CT (computed tomography)
    a. Bony tissue
    b. Soft tissue
19
Q

What does an ECG measure?

A

Electrocardiogram: measure electrical activity in the heart

20
Q

What’s the difference between fecal flotation and fecal sedimentation?

A

Fecal flotation:
* Salt or sugar solutions to “float” the parasites
* Parasites and parasite ova

Fecal sedimentation:
* For ova or other things that are too large to float
* Typically fluke eggs, larvae

21
Q

What three things are commonly measure in serologic tests? What 4 types of fluids can be used?

A

3 types of commonly serologic tests measure:
1. Antigen
2. Antibodies
3. Hormones

4 types of fluids:
1. Fecal
2. Saliva
3. Whole blood
4. urine

22
Q

what are the principles behind the direct and indirect immunofluorescent/immunoenzymatic tests?

A

Indirect immunofluorescent: a 2-step serological technique for the detection of circulating antibodies in body fluids, using 2 antibodies

Direct immunofluorescent: uses a single antibody directed against the target of interest

Indirect immunoenzymatic: intracellular detection of antibodies

Direct immunoenzymatic: require primary antigens to detect the antibodies

23
Q

What is reverse-transcriptase PCR used to detect?

A

Nucleic acid from the SARS- coV-2 in upper respiratory specimens

24
Q

what are the typical steps of PCR?

A
  • Sampling
  • DNA/RNA extraction
  • Amplification
  • Interpreting results
25
Q

what are the components of PCR?

A
  • Master mix
  • Primers
  • Molecular grade water
  • Extracted nucleic acid (RNA, DNA) from the sample
26
Q

describe a visualization method of PCR

A

Gel electrophoresis: used to separate mixtures of DNA, RNA, and proteins according to molecular size

27
Q

difference between sensitivity and specificity?

A

Sensitivity: ability of the test to diagnose a true positive

Specificity: ability of the test to diagnose a true negative

28
Q

Define true positive, true negative, false positive, and false negative

A

True positive: correctly identified and confirmed for a sickness

True negative: correctly identified for someone to not have any remanence of sickness

False positive: outcome where the test incorrectly predicts the positive class

False negative: outcome where the test incorrectly predicts the negative class

29
Q

Be able to draw the sensitivity and specificity 2x2 square and use the equations to solve for sensitivity and specificity of diagnostic tests

A

Test values Diseased Non-diseased
Positive True positive False positive
Negative False negative True negative