Concurrent #2 Better Flashcards
Requires oxygen, oxidase positive
obligate aerobe
Killed upon exposure to oxygen and has fermentation metabolism
obligate anaerobe
Can grow either aerobically, using oxidative phosphorylation or anaerobically
Facultative anaerobe
Requires oxygen for growth but prefers a reduced O2 environment
Microaerophilic bacteria
Prefer Co2 concentrations between 3-10%
Capnophilic bacteria
Most bacterial pathogens can utilize carbohydrates as carbon and energy sources via fermentation and/or oxidative catabolism
Saccharolytic
Same bacteria can not use carbohydrates as energy sources but can derive energy by oxidation of other types of organic compounds like amina acids, organic acids, and alcohols
asaccharolytic
Ex of obligate aerobe
Mycobacterium
Ex of obligate anaerobe
Clostridium, bacteroides
Ex of facultative anaerobe
E. coli, staphylococcus
Ex of microaerophilic
Borrelia
Ex of capnophilic
Brucella
Ex of asaccharolytic
Campylobacter, leptospira, rickettsia
E. Coli, Salmonella, C. Perfringens, Mycobacterium paratuberculosis
Diarrhea causing pathogens in cattle
Obligate aerobic, facultative anaerobic, obligate anaerobic
Oxygen requirement
Different types of media
Nutritional requirement
Blood agar media
Non selective media
MacConkey Agar and Mannitol salt agar
Selective agar media
Contains bile salts for enteric bacterial growth
MacConkey agar
High salt for staphylococci growth
Mannitol salt agar
Stains mycobacterium
Acid-fast stain
Differentiates e. coli and salmonella
Lactose test
Differentiates Gram positive bacteria
Catalase Reaction (+ bubbles)
Differentiates Gram negative bacteria
Oxidase Reaction (- causes color change)
K antigen
capsule
O antigen
LPS
H antigen
flagella
Rickettsia and Chlamydia tests
Tests for obligate intracellular bacteria
Sterilizing gas (ethylene oxide) and halogens (iodine)
Chemical agent of sterilization
A single dose can induce lifelong immunity
Live attenuated bacterial vaccine
B. abortus strain RB 51
Live attenuated vaccine
Oral for enteric bacteria
Live attenuated vaccine
Whole killed bacteria
Bacterins
Examples: C. chauvoei, C septicum, campylobacter fetus, brucella, potomac horse fever
Bacterin (whole killed vaccine)
Examples: C. tetani and Cornynebacterium diphtheria
Toxoid
Inactivated bacterial exotoxin
Toxoid