Cleaning, disinfection and sterilisation Flashcards

1
Q

How can we keep patients safe?

A
  • Infection policy for different organisms - details mode of transmission etc and must be reviewed frequently
  • Public awareness about washing hands etc
  • Wash hands
  • Sterilise
  • PPE
  • Sterilize touch-points
  • MRSA swab
  • Hand gel
  • Autoclave - sterilize with heat and pressure
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2
Q

When should you be cleaning hands?

A
Before patient contact 
Before clean/aseptic procedure
After bodily fluid exposure risk
After patient contact
After contact with patient surroundings
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3
Q

How do bacteria divide?

A

Binary fission

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4
Q

What do biofilms do?

A

Protect bacteria against desiccation and chemical attack

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5
Q

Examples of bacterial spores

A

Clostridium and bacillus spp

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6
Q

Why are spores beneficial ?

A

Survive adverse conditions and can survive if the bacterium itself dies

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7
Q

What are spores?

A
  • Allow bacteria to exist in dormant state
  • Complex, multi-layered coat that is resistant to heat, drying, alcohol and UV
  • Bacteria are able to return to a vegetative state when conditions are favorable
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8
Q

Characteristics of a virus

A
  • Capsid: made of nucleic acid and protein coat

- Some viruses have a lipid envelope that originates from the host cell

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9
Q

Is the lipid coat strong?

A

No, fragile and susceptible to heat, drying and chemicals

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10
Q

Are non-enveloped viruses tougher than enveloped ones?

A

Yes

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11
Q

What are dirty hands and how should they be cleaned?

A

Exposure to bodily fluids, using soap and water

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12
Q

What are clean hands and how should they be cleaned?

A

Examining patients etc, using alcohol hand gel

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13
Q

What is sterile equipment?

A

Introduced into sterile body area or into break in body membrane, e.g. gloves/cannula

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14
Q

What is clean equipment?

A

Not coming into close contact with mucus membrane or sterile body areas, e.g. ear scope, stethoscope

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15
Q

What is disinfected equipment?

A

Destroys harmful pathogens on surfaces, e.g. endoscope

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16
Q

Which pathogen is commonly associated with urinary catheters?

A

Pseudomonas

17
Q

What is sterilisation?

A

Destroying all micro-organisms and spores

18
Q

How do you sterilise something?

A
  • Best method is heat and pressure in an autoclave
  • 15 minutes at 121-124 degrees and 200kPa pressure
  • Blast with gamma rays
  • Filter through super fine filters
  • Gas (ethylene oxide)
19
Q

Which gas is used to sterilise?

A

Ethylene oxide

20
Q

What radiation is used to sterilise?

A

Gamma rays

21
Q

Conditions for autoclave

A

15 minutes at 121-124 degrees and 200 kPa pressure

22
Q

Why can disinfection be uncertain?

A
  • Composition of the surface to be disinfected can change
  • Presence of differing organic material
  • Type and number of organisms
  • Concentration of disinfecting agent, contact time
  • Temperature
  • pH
23
Q

What is antisepsis?

A

Disinfecting living tissue by using antiseptics

24
Q

Common disinfectants

A

Alcohol, chlorhexidine, iodine/chlorine, hydrogen peroxide vapor

25
Q

What is disinfecting?

A

Killing harmful microorganisms on non-living surfaces

26
Q

Characteristics of disinfection/antisepsis

A

Eliminating defined scope of microorganisms
Doesn’t eliminate most bacteria spores
Doesn’t necessarily kill bacteria