Chapter 8 - Microscopy and Lab Techniques Flashcards

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1
Q

Fixation

A

process of getting cells to stick on a microscope slide

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2
Q

Heat fixation

A

Cell placed on one side of microscope slide, heat applied with Bunsen burner, cells are stained

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3
Q

Optical microscopy

A

shine light on a sample, light reflects and pass through series of lenses which magnify the object; can be used to observe living cells

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4
Q

Electron microscopy

A

bombard sample with electrons, electrons bounce off the sample and pass through series of magnetic fields and land on a screen. Viewed on a computer (Indirectly viewing)

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5
Q

Electron microscopy requires cells to be:

A

fixed, stained, and killed before being observed

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6
Q

Which offers higher resolution - electron or optical microscopy?

A

Electron microscopy

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7
Q

Stereo microscope

A

low magnification to observe the surface of live specimens

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8
Q

Compound microscope

A

more than one lens; can be used to view simple, one-cell thick live cells

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9
Q

Drawback of compound microscopy

A

poor contrast - however, fixing/staining the cell would kill it

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10
Q

Bright field microscopes

A

compound microscopes that have a bright light to illuminate the sample

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11
Q

Phase contrast microscopes

A

allow the visualization of thin samples containing live cells; have tremendous contrast abilities

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