Chapter 2: 2.2

Question 1

What is magnification?

Answer 1

How many times larger the object is than its’ actual size of the image

Question 2

What is the function of the interchangeable objective lens?

Answer 2

Allows users to adjust resolution on a compound light microscope

Question 3

How does resolution affect microscope performance?

Answer 3

Determines the amount of detail that can be seen

Question 4

What is resolution?

Answer 4

The ability to see individual objects into separate entities

Question 5

How does diffraction affect resolution?

Answer 5

When light passes through specimen it gets reflected by individual structures can overlap = meaning that structures are no longer seen as separate entities

Question 6

How do electron beams increase the resolution?

Answer 6

When electron beams get defracted, the shorter wavelengths can be much closer before the overlap = meaning objects are much smaller and closer together; and be seen separately without diffraction blurring the image

Question 7

What is the formula for magnification?

Answer 7

size of image
magnification = ————————————-
actual size of the object

Question 8

What causes resolution in an optical microscope to become limited?

Answer 8

microscopy structures are closer than the half the wavelength of light cannot be seen separately