Chapter 13.3.1 (Exam 3) DNA Replication

Question 1

What are the three steps in DNA replication?

Answer 1

Initiation

Elongation

Termination

Question 2

What is initiation in replication?

Answer 2

double helix is unwound, making two template strands

Question 3

What is elongation in replication?

Answer 3

addition of complementary base pairs linked by phosphodiester bonds

Question 4

What is termination in replication?

Answer 4

DNA synthesis ends when all DNA regions have been replicated

Question 5

What are the monomers that are added to DNA during elongation?

Answer 5

deoxyribonucleoside triphosphates (dNTPs)

Question 6

Which end are the dNTPs added to?

Answer 6

3’ end

Question 7

What bond is formed between two nucleotides?

Answer 7

Phosphodiester bonds

Question 8

What provides the energy for the formation of phosphodiester bonds?

Answer 8

Release of phosphates from dNTPs

Question 9

When does DNA replication start?

Answer 9

DNA replication starts when a large protein complex (pre-replication complex) binds to a region called origin of replication (ori)

Question 10

What happens during DNA replication in E. coli?

Answer 10

In E. coli, DNA is unwound and replication proceeds in both directions, forming two replication forks