CHAP 10 SUMMARIZED Flashcards
Labeling Requirements for Blood and Components
• (?) symbology
• (?) of product
• (?) of preparation (whole blood, apheresis)
• Storage (?)
• Preservatives/(?)
• (?) in pooled components
• (?) of collection & processing facility
• (?) (& time if applicable). When expiration time not indicated, expiration is at midnight.
• Identification number for unit or pool
• Donor category (volunteer, autologous)
• (?), if applicable.
• (?)information
• (?) regarding recipient identification, Circular of Information, infectious disease risk, & prescription requirement
• (?): “For Autologous Use Only.”
Biohazard label if any infectious disease markers are pos or “Donor Untested” if testing not performed.
ISBT 128 bar-code
Name
Method
temperature
anticoagulant
Number of units
Name, address, registration number, & license number
Expiration date
ABO group & Rh type
Special handling
Statements
Autologous units
Rapid blood loss
Whole blood
Neonatal exchange transfusion
Whole blood
Symptomatic anemia
Packed RBCs
Avoid febrile or allergic transfusion reactions
Deglycerolized RBCs
Washed RBCs
WBC antibodies
Leukocyte-poor RBCs
DIC
FFP
Platelets
Vitamin K deficiency
FFP
Massive transfusions
FFP
Thrombocytopenia
Platelets
ITP
Platelets
Volume expander
Plasma
Hemophilia A
Cryoprecipitate
Factor XIII deficiency
Cryoprecipitate
Hypofibrinogenemia
Cryoprecipitate
Neutropenia
Granulocytes
Sepsis
Granulocytes
Separated from WB by centrifugation or sedimentation any time before expiration date of WB, or collected by apheresis.
RBCs
Additive solution added to RBCs following removal of most plasma.
RBCs adenine, saline added
Frozen in glycerol within 6 days of collection.
RBCs frozen
High glycerol (40%) method most commonly used.
RBCs frozen
RBCs washed with saline.
Washed RBCs
Filtration or apheresis processing.
RBCs leukocytes reduced
Irradiation at 2,500 cGy.
RBCs irradiated
Plasma separated from WB & frozen within 8 hr of collection
Fresh frozen plasma (FFP)
Prepared by thawing FFP at 1°–6°C, removing plasma, & refreezing within 1 hr
Cryoprecipitate
Centrifugation of WB at RT within 8 hrof collection.
Platelets
Apheresis.
Apheresis platelets
WBCs removed by filtration or during apheresis processing.
Leukocytereduced platelets
4–6 ABO-identical plt pooled using closed system.
Pre storage pooled platelets
1°–6°C
RBCs
RBCs adenine, saline added
Washed RBCs
RBCs leukocytes reduced
RBCs irradiated
Frozen in high glycerol: ≤65°C.
RBCs frozen
After deglyc (washing in decreased concentration of saline): 1°6°C
RBCs frozen
Frozen: ≤-18°C.
Fresh frozen plasma (FFP)
Cryoprecipitate
After thawing: 1°-6°C
Fresh frozen plasma (FFP)
After thawing: RT
Cryoprecipitate
20°–24°C
Platelets
Apheresis platelets
Leukocytereduced platelets
Pre storage pooled platelets
35 days in CPDA-1
RBCs
42 days
RBCs adenine, saline added
Frozen: 10 yr.
RBCs frozen
After degylc: 24 hr (unless closed system used)
RBCs frozen
24 hr after washing
Washed RBCs
Closed system: same as RBCs.
RBCs leukocytes reduced
Open system: 24 hr
RBCs leukocytes reduced
Original outdate or 28 days from irradiation, whichever comes 1st
RBCs irradiated
Frozen: 12 mo.
Fresh frozen plasma (FFP)
Cryoprecipitate
After thawing: 24 hr
Fresh frozen plasma (FFP)
After thawing: single units 6 hr, pools 6 hr if sterile connecting device used, other- wise 4 hr
Cryoprecipitate
5 days from collection with agitation.
Platelets
Apheresis platelets
After pooling, 4 hr
Platelets
Open system—4 hr.
Leukocytereduced platelets
Apheresis— 5 days
Leukocytereduced platelets
5 days from collection
Pre storage pooled platelets
Inadequate tissue oxygenation
RBCs
Same
RBCs adenine, saline added
RBCs frozen
Apheresis platelets
Pre storage pooled platelets
History of severe allergic rxn (e.g., IgA, other plasma proteins)
Washed RBCs
History of febrile rxn
RBCs leukocytes reduced
Immunodeficiency, malignancy, bone marrow transplant, tf with blood from blood relative, intrauterine & neonatal tf
RBCs irradiated
Deficiency of coag factors
Fresh frozen plasma (FFP)
Fibrinogen & factor XIII deficiencies
Cryoprecipitate
Severe thrombocytopeniaor abnormal platelet function
Platelets
Recurrent febrile rxn & to decrease risk of CMV transmission or HLA alloimmunization
Leukocytereduced platelets
Should have HCT ≤80%; otherwise not enough preservative to support RBCs.
RBCs
1 unit should increase HGB 1 g/dL or HCT 3%.
RBCs
Most commonly used RBC product.
RBCs adenine, saline added
Osmolality to monitor glycerol removal.
RBCs frozen
Virtually all plasma, anticoagulant, WBCs, & platelets removed.
RBCs frozen
Safe for IgAdeficient pt.
RBCs frozen
Used to store rare cells.
RBCs frozen
Not a substitute for leuko- reduced RBCs.
Washed RBCs
About 20% of RBCs lost in process.
Washed RBCs
Must retain 70- 85% of original RBCs. <5 × 106 WBCs.
RBCs leukocytes reduced
For prevention of graft-vs.-host disease.
RBCs irradiated
Kills donor T cells.
RBCs irradiated
Contains all coag factors.
Fresh frozen plasma (FFP)
Check for evidence of thawing & refreezing.
Fresh frozen plasma (FFP)
Thawed at 30°–37°C or by FDA-approved microwave.
Fresh frozen plasma (FFP)
Used for hemophilia A & von Willebrand disease only if factor VIII concentrate or recombinant factor preparations not available.
Cryoprecipitate
Should contain ≥80 IU of factor VIII & ≥150 mg of fibrinogen.
Cryoprecipitate
40–70 mL plasma.
Platelets
pH ≥6.2.
Platelets
1 unit should increase pltby 5,000–10,000/μL in 75-kg recipient.
Platelets
Shouldn’t be used if visible aggregates present.
Platelets
May contain residual RBCs.
Platelets
Usually pooled (4–6 units).
Platelets
≥5.5 ×1010 plt.
Platelets
≥3.0 ×1011 plt.
Apheresis platelets
Equiva-lent to 4–6 units.
Apheresis platelets
Exposes recipient to fewer donors.
Apheresis platelets
Extended outdate over-comes need for tfservice to pool just before administration.
Pre storage pooled platelets
1st soft spin yields platelet-rich plasma. 2nd hard spin separates pltfrom plasma.
Platelets
