Ch.5 Microbial Metabolism Flashcards
Big Picture: Metabolism
~ The — and — of any microorganism in a given environment depend on its metabolic characteristics
1. The sum of all — reactions within a living organisms is known as metabolism
2. Catabolism refers to chemical reaction that result in the — of more complex organic molecules into simpler substances. Catabolic reactions usually — energy
3. Anabolism refers to chemical reactions in which simpler substances are — to form more complex molecules. Anabolic reactions usually ——
- Survival & Growth
- Chemical
- Breakdown
- Release
- Combined
- Require Energy
Big Picture: Metabolism
~ Metabolism is the — and — of nutrients within a cell
~ These chemical reactions provide — and create substances that — life
- Buildup
- Breakdown
- Energy
- Sustain
Big Picture: Metabolism
~ Although microbial metabolism can cause — and ——, many pathways are beneficial rather than —
- Disease
- Food Spoilage
- Pathogenic
Catabolic and Anabolic Reactions:
~ Metabolic pathways are sequences of —— chemical reactions in a cell
~ Metabolic pathways are determined by —
* Enzyme names usually end in —
~ Enzymes are ——
~ Catalysts —— chemical reactions without being altered
~ Enzymes act on a — — and lower the ——
- Enzymatically catalyzed
- Enzymes
- Ase
- Biological Catalysts
- Speed up
- Specific Substrate
- Activation Energy
Factors Influencing Enzyme Activity:
1. —
2. —
3. ——
4. —
- Temperature
- pH
- Substrate Concentration
- Inhibitors
Factors Influencing Enzyme Activity:
~ —— and — pH denature proteins
~ If the concentration of the substrate is high (—), the enzyme catalyzes at its — rate
- High Temperature
- Extreme
- Saturation
- Maximum
Oxidation-Reduction Reactions:
~ Oxidation: — of electrons
~ Reduction: — of electrons
~ Each time a substance is oxidized, another is simultaneously—
~ Redox Reaction: an — reaction paired with a — reaction
- Removal
- Gain
- Reduced
- Oxidation
- Reduction
Oxidation-Reduction Reactions
~ In biological systems, electrons and proton are removed at the — time; equivalent to a — atom
~ Biological oxidations are often —
- Same
- Hydrogen
- Dehydrogenations
The Generation of ATP:
~ ATP is generated by the — of — with the input of —
~ Why is glucose such an important molecule for organisms?
* Glucose is a — molecule; energy is — during a cell’s — of —
- Phosphorylation of ADP
- Energy
- Reduced
- Released
- Oxidation of Glucose
Substrate-Level Phosphorylation:
~ ATP generated when — energy — added to ADP generates —
* Occur during — and ——
- High
- PO4
- ATP
- Glycolysis
- Kreisler Cycle
Oxidative Phosphorylation:
~ Electrons are transferred from one electron carrier to another along ——— (system) on a membrane that — energy to generate —
- Electron Transport Chain
- Releases
- ATP
Metabolic Pathways of Energy Production:
~ Series of enzymatically catalyzed chemical reactions
~ Extracts energy from —— and stores in in chemical form(—)
~ Most of a cell’s energy is produced from oxidation of —
~ The two major types of glucose catabolism are —, in which glucose is completely broken down, and —, in which it is partially broken down
- Organic Compounds
- ATP
- Carbohydrates
- Respiration
- Fermentation
Aerobic Respiration:
~ The breakdown of — to — energy
* Glycolysis: —
* Krebs Cycle: —
* Electron transport chain(system): —— of —
~ The oxidation of glucose to pyruvic acid produces — and — during glycolysis
* OVeral net gain of — molecules of — for each molecule of glucose oxidized
* —— phosphorylation
- Carbohydrates
- Release
- Cytosol
- Mitochondria
- Inner membrane of mitochondria
- Two
- ATP
- Substrate-level
Glycolysis:
~ — stage
* —— are used
* Glucose is split to form two molecules of ———
- Preparatory
- 2 ATP
- Glyceraldehyde 3-phosphate
Aerobic Respiration:
~ ——
* —— (from glycolysis) is oxidized and — (loss of CO2) occurs
* THe resulting two carbon compound attaches to ——, forming —— and —
* Oxidation of acetyl CoA produces —, —, and —, and liberates — as waste
* Substrate-level phosphorylation
- Krebs Cycle
- Pyruvic Acid
- Decarboxylation
- Coenzyme A
- Acetyl CoA
- NADH
- NADH, FADH2, and ATP
- CO2