CH2 magnification and calibration Flashcards
1
Q
What is magnification
A
How many times larger the image is than the actual size of the object being viewed
2
Q
What is resolution
A
The ability to see individual objects as separate entities
3
Q
What is resolution limited by
A
The diffraction of light as it passes through samples
4
Q
What is diffraction
A
The tendency of lightwaves to spread as they pass close to physical structures
5
Q
How do you calculate magnification
A
Image size/actual size
6
Q
What is an eyepiece graticule
A
- glass disc marked with a fine scale of 1 to 100
- scale has no units
7
Q
What is a stage micrometer
A
A microscope slide with a very accurate scale in micrometers engraved on it