ch16+17 Flashcards
reflectance photometry
quantifies the intensity of colored product produced on reagent strip reaction pads
semiautomated vs fully automated urine chemistry analyzers
semi: user properly digs reagent strip and place in on platform, instrument automatically reads pads at time
fully: user places labeled tubes of urine into rack and instrument fully performs test
advantages gained by performing automated urine sediment analysis
elimination of observation associated variation, reduce need for manual microscopy review
digital flow morphology
Iris iQ200 –>capture and automatically classify digital images of urine particles as they pass flowcell
flow cytometry
UF-5000 –> urine particles are identified and categorized based on forward scatter
digital microscopy
UriSed 2 –> scans for urine particles at single focal plane of cell
dilutents
isotonic saline –> normal salines for cell counts
dilute acetic acid –> lyses RBCs
step by step how to perform a manual cell count
pipette diluted body fluid into hemacytometer
allow to settle
examine for even distribution
less than 200 cells present
count all 9 squares
more than 200 cells present
count 4 large corner squares
more than 200 cells in 1 large square
count all five RBC squares