ch 12

Question 1

what and where are sperm cells regulated

Answer 1

sertoli cells in seminiferous tubules

Question 2

specimen collection purpose

Answer 2

assess male fertility

Question 3

when should specimen collection take place

Answer 3

within 3 months at least 7 days apart and sexual abstinence for 2 days

Question 4

what temp should sperm be kept

Answer 4

at room temp or close to body temp to prevent cold shock

Question 5

physical examination

Answer 5

appearance: gray-white and opalescent

Question 6

motility grade 1

Answer 6

motile without forward progression

Question 7

motility grade 2

Answer 7

motile with slow nonlinear progression

Question 8

motility grade 3

Answer 8

motile with moderate linear progression

Question 9

motility grade 4

Answer 9

motile with strong linear progression

Question 10

normal sperm concentration

Answer 10

20-250 million per mL

Question 11

how is concentration measured

Answer 11

by using hemacytometer after 1;20 dilution is prepared

Question 12

sources of error with sperm

Answer 12

counting and high concentration

Question 13

morphology of sperm

Answer 13

head, midpiece, and tail

Question 14

vitality

Answer 14

differentiation of live and dead sperm
–> alive does not pick up stain, while dead does

Question 15

agglutination

Answer 15

clumping of motile sperm
ex. immunoglobulin G and A antibodies

Question 16

ph of semen

Answer 16

7.2 to 7.8

Question 17

fructose

Answer 17

presence reflects secretory function of seminal vesicles (13 umol)
–> low fructose = azoospermia

Question 18

citric acid

Answer 18

decreased levels indicate dysfunction of prostate gland (52 mmol)

Question 19

acid phosphate

Answer 19

asses secretory function of prostate gland
–> used in rape and sexual assult cases to identify semen