Ch. 17 Control of Gene Expression in Eukaryotes

Question 1

Characteristics of eukaryotic gene expression

Answer 1

1 promoter for 1 gene
DNA must unwind from histones
transcription and translation are separated

Question 2

acetylation of histones ____ transcription

Answer 2

increases

Question 3

the enzyme that adds acetyl groups to histones

Answer 3

acetyltransferase

Question 4

deacetylase ____ transcription

Answer 4

decreases

Question 5

CpG islands are found on

Answer 5

DNA

Question 6

in CpG islands, which nucleotide is methylated

Answer 6

C

Question 7

methylation in CpG islands ____ transcription

Answer 7

decreases

Question 8

where is the best place for CpG islands?

Answer 8

near the transcription start sites

Question 9

genomic imprinting is an example of…

Answer 9

methylation

Question 10

this DNA sequence causes transcription to increase

Answer 10

enhancer

Question 11

these DNA sequences serve to block the effects of enhancers

Answer 11

insulator

Question 12

True or False: if no insulator are present, one enhancer sequence could regulate any genes

Answer 12

True

Question 13

insulators create neighborhoods of gene regulation called

Answer 13

topographically associated domain

Question 14

which two RNA’s are important in silencing and gene regulation?

Answer 14

siRNA
miRNA

Question 15

this enzyme breaks down double-stranded RNA

Answer 15

dicer

Question 16

what is the purpose of dicer?

Answer 16

make siRNA or miRNA

Question 17

True or False: RISC is a complex of proteins and RNA

Answer 17

True

Question 18

what does RISC stand for?

Answer 18

RNA-induced silencing complex

Question 19

which type of RNA pairs perfectly with mRNA

Answer 19

siRNA

Question 20

what type of RNA is made from cutting up a hairpin?

Answer 20

miRNA

Question 21

siRNA + RISC causes

Answer 21

RNA cleavage

Question 22

miRNA + RISC causes

Answer 22

inhibition of translation
RNA cleavage

Question 23

transcriptional silencing uses

Answer 23

siRNA + RITS