Ch. 12 DNA Replication and Recombination Flashcards
Replication results in a random mix of old and new DNA in two daughter DNA’s
dispersive
Replication results in one completely new DNA and one completely old DNA
conservative
Replication results in two DNA molecules where each molecule has one old and one new strand
semiconservative
How does DNA replicate?
semiconservative
The location where DNA begins
origin of replication
Has one replication bubble?
theta
Has many replication bubbles?
linear
Used for circular chromosomes?
theta
Is linear replication unidirectional or bidirectional?
bidirectional
True or False: Eukaryotes use linear DNA replication
True
What are the requirements of replication?
single-strand DNA template
dNTP’s
enzymes
To which end of DNA is the new nucleotide added?
3’
What group at the 3’ is the most important?
OH
DNA is…
antiparallel
complementary
made from 5’ to 3’
What bond is in the backbone of DNA?
phosphodiester
Which direction is the template strand read?
3’ to 5’
Which direction is the new strand being made?
5’ to 3’
leading strand=
continuous
lagging strand=
discontinuous
If the new strand goes 5’ to 3’ into the fork…
leading
If the new strand goes 5’ to 3’ away from the fork…
lagging
What small fragments are formed on the lagging strand?
Okazaki
What enzyme adds new nucleotides to the DNA strand?
DNA polymerase
What enzyme fuses the Okazaki fragments?
ligase