cell cycle & mitosis - exam 2 Flashcards
diploid
cells/organisms w/ paired chromosomes
2n
haploid
cells/organisms w/ unpaired chromosomes
1n, n
structure of eukaryotic chromosomes
gene
dna
nucleosomes
30nm fibers
looped domains
metaphase
sister chromatids
replicated chromosomes
cohesions
glue like proteins connecting sister chromatids along their length
centromere
region where sister chromatids attach
kinetochore
protein structure where MTs attach during mitosis
metaphase
tightest chromosome packing
right before cell division begins
G0
resting stage
diploid cell
G1
cell growth, transcription/translation
duplication of organelles
prepare for dna replication
S
dna replication
G2
more growth
transcription/translation
prepare to divide: centrosomes appear
centrosome
microtubule organizing center
M
mitosis - divided into 4 stages
interpahse
G1 + S + G2
why do microtubules grow & shrink during cell divison
kinetochore MTs shrink to pull chromosomes apart
nonkinetochore MTs grow to push the cell apart
late interphase cell
2n =4
centrosomes together
replicated dna
prophase
chromosomes condense - metaphase
nuclear envelope degrades
centrosomes migrate to opposite poles of the cell
mitotic spindle forms & MTs
mitotic spindle
centrosomes + microtubules that grow from it
kinetochore MTs
attach to kinetochores of each chromosome
nonkinetochore MTs
do not attach to the kinetochores
metaphase
chromosomes line up on metaphase plate
4 chromosomes - each composed of 2 sister chromatids = total of 8 sister chromatids
metaphase plate
hypothetical plate on halfway point between centrosomes
anaphase
separation of dna
cohesions degrade
kinetochore MTs get shorter
sister chromatids separate
nonkinetochore MTs lengthen –> cell elongates
motor proteins push against each other to elongate cell
8 chromosomes
0 sister chromatids
telophase / cytokinesis
chromosomes decondense
nuclear envelope reforms
centrosomes & spindles disappear
microfilaments pinch cleavage furrow into the cell splits
2 2n=4 cells
binary fission
copy dna
separate dna
binary fission process
1 chromosome + 1 replication bubble
replicates into 2 chromosomes
contractile ring gets tighter until cell splits
2 cells