Carbohydrates 3 Flashcards

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1
Q

Where does glycolysis occur?

A

Cytosol

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2
Q

Preparatory phase

A

1 glucose –> 2 G3P

2 ATP used

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3
Q

What steps are irreversible?

A

1,3 and 10

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4
Q

Catalyst of step 1

A

Hexokinase

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5
Q

After what step are you committed to glycolysis?

A

3

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6
Q

Step 10

A

PEP–> pyruvate using pyruvate kinase

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7
Q

Where is NADH produced?

A

Steps 6 - 2NADH

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8
Q

Where is ATP produced?

A

Step 7 - 2ATP and 10 - 2ATPFates

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9
Q

What steps are coupled?

A

6 and 7

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10
Q

What is glucose split into first?

A

Fructose-1,6- BP

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11
Q

Fructose -6-phosphate to fructose-1,6-BP

A

Phosphofructokinase 1

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12
Q

Substrate level phosphorylation

A

Requires soluble enzymes and chemical intermediates

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13
Q

Respiration-linked

A

Membrane bound enzyme and gradients of protons

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14
Q

Redox balance

A

NAD+ ESSENTIAL for glycolysis but limited in a cell so must be replenished to reduce intermediates

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15
Q

Fates of pyruvate

A
  • ethanol
  • lactate
  • acetyl coA
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16
Q

Pyruvate–> ethanol

A
  • Pyruvate decarboxylase
  • Alcohol dehydrogenase
  • NAD+ made and used in glycolysis
17
Q

Pyruvate -> lactate

A

Exercising cells and also replenishes NAD+

Replenish oxygen debt by cori cycle

18
Q

Pyruvate-> acetyl coA

A

in mitochondria