Blood & Hematocrit (LAB PRACTICAL) Flashcards
Erythrocytes
-Red blood cells that use hemoglobin to carry O2 to blood tissues
-Most abundant formed element
Leukocytes
White blood cells
Granulocytes
Leukocytes with visible granules in their cytoplasm
-Neutrophils, eosinophils, basophils
Agranulocytes
Leukocytes without visible granules in their cytoplasm
-Lymphocytes & Monocytes
Diapedesis
Process of white blood cells leaving capillaries onto surrounding tissue.
Platelets
Fragments of megakaryocytes that aid in blood clotting
Physical Description of Neutrophils
-Multilobed nucleus
-Pale red and blue cytoplasmic granules
-10 to 12 um diameter
Physical Description of Eosinophil
-Bi-lobed nucleus
-Red cytoplasmic granules
-10 to 14 um diameter
Physical Description of Basophils
-Lobed nucleus
-Large blue & purple cytoplasmic granules
-10 to 14 um diameter
Physical Description of Lymphocytes
-Spherical or indented nucleus
-Pale blue cytoplasm
-5 to 17 um diameter
Physical Description of Monocyte
-Nucleus U or kidney-shaped
-Gray or Blue cytoplasm
-14 to 24 um diameter
Function of neutrophils
phagocytize pathogens and debris
Function of eosinophils
-Kill parasitic worms
-Slightly phagocytic
-Complex role in allergy and asthma
Function of basophils
-Release histamine and other mediators of inflammation
-Contains heparin (Anticoagulant)
Function of lymphocytes
Mount immune response by direct cell attack or via antibody production
Function of monocytes
Develop into macrophages in tissues and phagocytize pathogens or debris
Function of platelets
Seal small tears in blood vessels
Performing a hematocrit
Place capillary tube into blood sample and measure (Height of RBC/Height of all blood components)
Order of blood centrifuged from top to bottom
- Plasma
- Buffy Coat
- Erythrocytes
Normal Hematocrit levels for Males, Females, and Newborns
Male: 42-52%
Female: 37-47%
Newborn: 49-61%
Leukocytosis
-Too high leukocyte count
-Buffy coat is over 1% of blood volume
-Caused by infection or some kind of leukemia
Anemia
Too low erythrocyte count
Aplastic anemia
Bone marrow does not produce enough erythrocytes
Iron-deficiency anemia
Erythrocytes are smaller
Hemolytic anemia
Erythrocytes are destroyed too quickly
Sickle cell anemia
Sickle-shaped erythrocytes
Hemorrhagic anemia
Caused by blood loss and not detected by hematocrit
Hematocrit of individuals living at high altitude
Higher hematocrit due to the lower levels of oxygen that will cause kidneys to produce more RBC’s
Affect of dehydration on anemia
Loss of water means less plasma