Bacterial Structure and Classes Flashcards
On Gram + induces TNF-a, IL-1 and IL-6
Lipoteichnoic acid
Gram stains purple
Gram +
Gram stains pink
Gram -
On Gram - induces TNF & IL-1
Lipid A portion of Lipolysaccharide
On Gram - Antigen
O polysaccharide portion of LPS
On Gram - site of endotoxin
LPS
In Gram - site of B-lactamases
Periplasmic space
Mycolic acid cell wall rich in lipids
Mycobacteria
Resistant to gram stain
Giemsa stain
"Certain Bugs Really TRY my Patience" Chlamydiae Borrelia Rickettsiae Trypansosomes Plasmodium Histoplasma
Silver stain
Legionella
Pneumocystis and other fungi
H.pylori
No cell wall
Requires sterols
Mycosplasm
Ureasplasms
Pleomorphic gram -
Rickettsiae
Haemophilus
Chlamydiae
PAS stain
Glycogen
Ziehl-Neelsen stain
Acid fast stain Carbol fuchsin stain Nocardia Mycobacteria Cryptosporidium
Doesn’t gram stain
"These Microbes May Lack Real Color" Trepenoma (too thin) Mycobaccteria Mycoplasma Legionella Rickettsia Chlamydia
Obligate Intracellular
"stay inside when its Really CHilly and COld" Rickettsia Chlamydia Coxiella Rely on host ATP
India ink stain
Cryptococcus neoformans
also mucicarmine stains red
Eosin methylene blue agar (EMB)
E.coli
Turns colonies green metallic sheen
Charcoal yeast extract agar with cysteine and iron
Legionella
Sabouraud agar
Fungi agar
Chocolate agar Factors V (NAD+) Factor X (hematin)
H.influenzae