Autoab Flashcards
A positive autocontrol or dat may indicate
Autoab
If the pt has not been transfused the pt may have an
Autoab coating in their own cells
If pt has been transfused the pt may have an
Alloab coating transfused cells
Mini cold panels can be used to help identify
Cold autoab
Anti I is an autoab what are usually included
Cord cells
How to get a positive DAT
Test for invivo coating rbc with ab
Ahg is added to washed pt red cell ti detemine
This react at rt with not all panels cells and give a posituve autocontrol
Cold autoab
The dat usually pos with
Ati c3 AHG
Cold autoab due to
Mycoplasma pneumoniae, im, or cold agglutinin
If the ab remain, what can be performed
Peewarmed technique
Is another technique in whc the autoab is removed from the patient serum usung their own red cell
Autoadsorption
Red cells, serum, saline are incubated at 37 re combines
Prewarmed technique
More common that cold ab
Warm ab
Positive dat due to igg ab coating the red cell
Warm autoab
Majority of the oanel or screening cells will be positive
Warm autoab
The rh system seem to be main target altho other occur
Warm autoab
Used to remove ab
Eluate
Testing eluate is useful in investigation of
Hdn
Transfusion rxns
Autoimmune dse
How do u get a warm autoab
Idiopathic
Known disorder leukemia, preg, ID
Medication
Most common elution
Acid elution
It lowers ph, causing ab to dissociate
Acid elution
Dissolve bilipid layer of rbc
Organic solvents ether chloroform
Elution methods
Acid ekution
Organic solvents
Freeze thaw
Heat
Free ab from sensitized eed cell so that ab can be identified
Elution, dat is pos
