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CLS 306 > AHG > Flashcards

AHG Flashcards

1
Q

what is the antiglobilin test known as

A

Coombs test

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2
Q

what immunoglobin has to be on RBC for agglutination to be visible in a Coombs test

A

IgG

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3
Q

what animal does anti-human globulin come from

A

rabbits

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4
Q

what does AHG bind to during a Coombs test

A

human immunoglobulins

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5
Q

what kind of antiglobulin test measures in vivo sensitization of RBC

A

direct

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6
Q

what kind of antiglobulin test measures in vitro sensitization of RBC

A

indirect

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7
Q

what is an example of an indirect anti globulin test

A

ab screen test

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8
Q

what kind of sample is used in a indirect Antiglobulin test

A

serum

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9
Q

during a direct antiglobulin test what does the reagent detect

A

IgG or C’

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10
Q

in a direct antiglobulin test what are the RBC washed with

A

saline

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11
Q

what needs to be added if a neg Coombs test is received

A

checked cells

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12
Q

what do checked cells verify

A

adequate washing, Anti-human globulin was added and working

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13
Q

after checked cells are added and a pos test is recored what does this mean

A

test is valid

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14
Q

after checked cells are added and a neg test is recored what does this mean

A

test is invalid and must be repeated

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15
Q

if a pos direct antiglobulin test uses poly specific AHG what need to be done

A

repeat using monospecific AHG reagent

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16
Q

what can cause the AHG reagent to become contaminated

A

putting vial in pt sample

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17
Q

what is detected in an indirect antiglobulin test

A

irregular abs in recipient sera

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18
Q

what is another name for Coombs reagent

A

antihuman globulin

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19
Q

what color is poly specific AHG reagent

A

clear

20
Q

what does ploy specific AHG contain

A

abs to human IgG and human C3d

21
Q

what is an advantage of polyspecific AHG

A

detects C’ binding Abs on RBC

22
Q

what is an disadvantage of polyspecific AHG

A

detects C’ on cells that may not be clinically sig

23
Q

what is the color of IgG monospecific AHG

A

green

24
Q

what does IgG monospecific AHG contain

A

abs to human IgG only

25
Q

what color is C3d monospecific AHG

A

clear

26
Q

what size is C3d monospecific AHG

A

small

27
Q

what does C3d monospecific AHG contain

A

abs to human C3d only

28
Q

what is an advantage of monospecific AHG

A

fewer nuisance pos

29
Q

who regulates Coombs reagents

A

FDA

30
Q

what do CC reagent contain

A

regent RBC coated with IgG

31
Q

A description of the antiglobulin test is:

A

AHG reacts with human globulin molecules bound to RBCs

32
Q

Polyspecific AHG reagent contains:

A

Anti-IgG and anti-C3d.

33
Q

Monoclonal anti-C3d is Derived from

A

one clone of plasma cells.

34
Q

Which of the following is a clinically significant antibody whose detection has been reported in some instances to be dependent on anticomplement activity in polyspecific AHG?

A

anti-Jk a

35
Q

After the addition of IgG-coated RBCs (check cells) to a negative AHG reaction during an antibody screen, a negative result is observed. Which of the following is a correct interpretation based on these findings?

A

The antibody screen cannot be interpreted.

36
Q

RBCs must be washed in saline at least three times before the addition of AHG reagent to:

A

Remove traces of free serum globulins.

37
Q

An in vivo phenomenon associated with a positive is:

A

Maternal antibody coating fetal RBCs.

38
Q

False-positive DAT results are most often associated with:

A

Use of refrigerated, clotted blood samples in which complement components coat RBCs in vitro.

39
Q

Polyethylene glycol (PEG) enhances antigen-antibody reactions by:

A

Concentrating antibody by removing water.

40
Q

Solid-phase antibody screening is based on:

A

Adherence

41
Q

A positive DAT may be found in which of the following situations

A

HDFN

42
Q

What do Coombs’ check cells consist of?

A

Type O-positive cells coated with anti-D

43
Q

Which of the following IAT methods requires the use of check cells?

A

Manual tube method with albumin

44
Q

Which uncontrollable factor can affect AHG testing?

A

Incubation time

45
Q

Which would be the most efficient method for a laboratory staffed by medical laboratory technicians?

A

Polybrene

46
Q

A 27-year-old group O mother has just given birth to a group A baby. Since the mother has IgG anti-A, anti-B and anti-A, B in her plasma, which of the following methods and tests would be most effective at detecting the anti-A on the baby’s RBCs?

A

IAT using gel

CLS 306 (19 decks)

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