8.2 Flashcards
Leading strand
The replicated strand that goes towards the replication fork. Continuous.
Lagging strand
The replicated strand goes away from the replication fork. Discontinuous.
Semi-discontinuous.
DNA replication is semi-discontinuous because of how the leading strand is continuous and the lagging strand is discontinuous.
Semiconservative Replication.
How DNA replicates: Parent strands are inherited on first round, the next daughter strands inheriting from the first strands.
Okazaki fragments
The fragmented portions that make up the lagging strand.
Helicase
Unzips and unwinds the DNA
Polymerase 3
Adds new nucleotides to the strands
Polymerase 1
Removes RNA primers and replaces them with nucleotides.
Ligase
Joins the fragmented strands of the lagging strand to make it continuous.
Primase
Adds RNA primers to the strands.
Order of events
Helicase > Primase > Polymerase 3 > Polymerase 1 > Ligase
Meselson and Stahl’s experiments
An experiment by Meselson and Stahl in which they were trying to find how DNA replicated from one whole strand to the next. They concluded that DNA replicates semiconservatively