7.2 Flashcards
Enzyme
A protein that catalyzes chemical reaction
Substrate
The reactants that get catalyzed by an enzyme
Active site
The place where the substrates go in the enzyme to react
Activation Energy
The minimum energy requirement for a chemical reaction
Properties of Enzymes
- Reaction Specific
- Not consumed in a reaction (reusable)
- Made of Protein and, sometimes, RNA
- Affected by cellular conditions.
What happens to reaction rate as ___ goes up?
Enzyme Concentration: Increases and then tops out due to every enzyme being used up
Substrate Concentration: Increases and then tops out due to every substrate being used up.
Temperature: Increases and then decreases at maximum (bell curve) due to temperature causing enzymes to denature (different enzymes can have different limits of temperature)
pH: Same as temperature where enzymes denature due to pH levels
How can enzyme activity be maximized? Minimized?
Enzyme activity can be maximized by placing it in an environment with good pH and temperature and an efficient number of substrates.
We can minimize an enzyme by putting it in a place where it is just hot enough or just has enough pH to survive and substrate levels are inefficient (possibly just simply denaturing the enzyme).
Inhibition vs. Activation
Activation, and activators, maintain the shape of the enzyme to keep the enzymes functions. Inhibition changes the shape of the enzyme (or blocks the active site from substrates) in order to change the function, causing the enzyme’s original substrate to not react with the enzyme.