8.2 Flashcards
leading strand
DNA enzyme is working towards the fork
lagging strand
DNA enzyme is working away from fork
semi-discontinuous
half continuous and half discontinuous
semi-conservative replication
okazaki fragments
fragments of added nucleotides not connected
what’s the function of helicase
breaks apart hydrogen bonds and separates the two DNA strands
what’s the function of primase
adds RNA primers so DNA III can add nucleotides
what’s the function of DNA polymerase III
adds nucleotides to the strands
what’s the function of DNA polymerase I
replaces primers with nucleotides and repairs and edits any mistakes
what’s the function of ligase
connects fragments
what’s the order of events in DNA replication
parental strand separated (helicase), adds RNA primers (primase), adds nucleotides (DNA polymerase III), replaces primers with nucleotides (DNA polymerase I), connects okazaki fragmets (ligase)
what was meselson and Stahl’s experiment
what was the conclusion of the experiment
the semi-conservative replication matched what they found originally
where are the leading and lagging strand on a diagram of DNA replication
whichever strand has the DNA III working towards the fork is the leading strand, and it will be one continuous piece without fragments. the lagging strand will have fragmented pieces and will be working away from the fork.
where are the 3’ and 5’ ends in a DNA replication diagram
the DNA strands are replicated from 5’ to 3’, so the new sides of the strands will be antiparallel to the parental strands and the 3’ and 5’ will be on opposite sides for the new parts.
where are the okazaki fragments in a DNA replication diagram
in the lagging strand
