2C Investigating Enzymatic Reactions

Question 1

How do you investigate the effect of ph on the rate of starch breakdown by amylase

Answer 1

• drop of iodine into well of sporting tile
• buses burner under beaker of water and heat until it’s 36’
• use syringe to add 1cm3 of amylase solution and 1cm3 of buffer solution with ph of 5 in boiling tube and place in beaker for 5 mins
• use diff syringe to add 5cm3 of starch into boiling tube mix and start stopwatch
• drop a fresh sample from boiling tube every 30 secs into well, when remain browny-orange the starch is no longer there

Question 2

How do you calculate the rate of chemical reaction

Answer 2

1000 divided by time