2.9 Proteomics Flashcards
What is proteomics?
the study of all the proteins that make up all your cells and tissues.
What is 1 and 2D electrophersis?
A way to combine these two ways, by separating them in the 1st dimension via iso electric charge via PH charge. Then the 2nd is by weight, allowing a more specific separation of all the proteins in the sample
What is the western blotting?
used to separate and identify proteins. In this technique a mixture of proteins is separated based on molecular weight, and thus by type, through gel electrophoresis. These results are then transferred to a membrane producing a band for each protein.
What are proteins?
Macromolecules consisting of one or more long chains of AA residues (polypeptides).
What three main classes can proteins be divided into?
globular, fibrous, and membrane
What is IEF?
Isoelectric focusing, technique for separating NONdenatured proteins by their isoelectric point and put them in a PH gradient and then separate on their accumulated charge based on their AA seqience
What is IEF?
Isoelectric focusing, technique for separating NONdenatured proteins by their isoelectric point and put them in a PH gradient and then separate on theiraccumulated charge based on their AA sequence
What is mass spec?
Breaks up proteins, into smaller pieces, gives them a charge, ionize it, run it across the detector that will detect how big that molecule is and create a unique pattern for each molecule and compare it to known patterns
What is the Yeast 2 hybrid technique?
Tool for examining the interactions between proteins. A way to search the genome to fish out transcription factors, dna factors.
What are protein microarrays?
The array has multiple antibodies on the plate itself, the proteins will stick and light up. Good tool to analyze the proteom for a particular sample.
What is affinity capture?
Method of separating chemical mixtures based on a highly specific interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. Good in purifying a protein.
Define the interactome/Reactome
Mostly protein protein interactions, the whole set of molecular interactions in a particular cell,
What is the importance of post-translational modification?
Increases protein diversity.
What are prions?
infectious angents, consequences of misfolded proteins that cause degeneration of brain matter. Tells other proteins to misfold.
What are Tau-plaques associated with?
Alzheimers
