1 lab exam material ( microscpy)

Question 1

Escherichia coli

Answer 1

gram negative

Question 2

Bacilluis subtilis

Answer 2

gram postive

Question 3

staphylococcus aureus

Answer 3

gram postive

Question 4

streptococcus pyogenes

Answer 4

gram postive

Question 5

Clostridium Botulinum

Answer 5

gram postive

Question 6

spirillum

Answer 6

gram negative

Question 7

Heat fix

Answer 7

allows the specimen or cells to stick to the slide, that way when staining afterwards the cells won’t wash them away. over heat fixing can kill the cells

Question 8

Simple staining

Answer 8

staining yeast cells with methylene blue

Question 9

Differential stains

Answer 9

use multiple stain that distinguish different microbial groups or structures and typically require a more complex procedure than simple stain

Question 10

examples of differential stains are what?

Answer 10

gram staining, acid fast staining, and endospore staining.

Question 11

Acid-fast staining

Answer 11

used to identify species that contain mycolic acids and other complex lipids in their cell walls.

Question 12

Endospore stainning

Answer 12

distinguishes endospores in sample from the vegtative cells

Question 13

postive stains

Answer 13

where the dyes used color the specimen

Question 14

negative stains

Answer 14

instead colors the background by adhering to the slide instead of the specimen, creating a silhouette against a darkbackground