02 PROTEINS in other body fluids

Question 1

A unique protein present only in multiple myeloma

Answer 1

Bence Jones proteins

Question 2

Bence Jones proteins are made up of ___ ___ chains

Answer 2

kappa/lambda light chains

Question 3

Bence jones proteins are soluble at ___C

Answer 3

100C

Question 4

Bence jones proteins precipitates at ___C

Answer 4

40-60C

Question 5

A unique protein found only in the urine

Answer 5

Tamm Horsfall protein

Question 6

Tamm horsfall protein is produced by the ___

Answer 6

renal tubular epithelial cells

Question 7

Normal range of Tamm horsfall protein

Answer 7

40 mg/day

Question 8

Indicator for the urine test strip for protein

Answer 8

Tetrabromphenol blue

Question 9

A color change in the urine test strip for protein is equivalent to ___ mg/dL of protein

Answer 9

more than or equal to 6 mg/dL

Question 10

In the turbidimetric method of proteins in other body fluids, this reagent produces 4 times more turbidity with albumin

Answer 10

Sulfosalicylic acid

Question 11

Reagents for the turbidimetric method of proteins in other body fluids

Answer 11

Sulfosalicylic acid
Trichloroacetic acid
Benzethonium chloride

Question 12

T/F: Folin Lowry method for the determination of proteins in other body fluids is 10x more sensitive than biuret method

Answer 12

T

Question 13

To increase the sensitivity of folin lowry method in determination of proteins in other body fluids, ___ is added as an initial step

Answer 13

biuret

Question 14

Adding biuret increases the sensitivity of folin lowry by ___x

Answer 14

100

Question 15

Accepted CSF protein level

Answer 15

15-45 mg/dL

Question 16

Protein in CSF is ___% that of plasma

Answer 16

<1%

Question 17

Reference value of CSF albumin to serum albumin

Answer 17

<2.7-7.3

Question 18

Increased CSF to serum albumin ratio is due to ___

Answer 18

damaged BBB

Question 19

Decreased CSF to serum albumin ratio is due to ___

Answer 19

fluid leak from CNS

Question 20

Normal CSF-IgG index

Answer 20

0.26-0.70

Question 21

Elevated CSF-IgG index may be indicative of ___

Answer 21

multiple sclerosis

Question 22

Protein unique to CSF

Answer 22

Tau protein/ Tau transferrin