01 BASIC PRINCIPLES AND PRACTICES Flashcards
This provides uniformity and consistency
SI units
Subclassifications of SI system
Basic units
Derived units
Selected accepted non-SI units
Give the SI unit:
Length
meter
Give the SI unit:
Mass
Kilogram
Give the SI unit:
Time
Second
Give the SI unit:
Electric current
Ampere
Give the SI unit:
Thermodynamic temperature
Kelvin
Give the SI unit:
Amount of substance
Mole
Give the SI unit:
Luminous intensity
Candela
Give the selected derived unit:
Frequency
Hertz
Give the selected derived unit:
Force
Newton
Give the selected derived unit:
Celsius temperature
Degree Celsius
Give the selected derived unit:
Catalytic activity
Katal
Give the selected non-SI unit:
Time
Minute, Hour, Day
Give the selected non-SI unit:
Volume
Liter
When is amniocentesis done?
2nd trimester
This is examined to determine the lung maturity of the fetus
Amniotic fluid
L:S ratio is used for which bodily fluid?
Amniotic fluid
These (2) are measured to determine the lung maturity of a fetus, which are important lung surfactants
Lectin
Sphingomyelin
The normal L:S ratio
> /= 2
Type of blood specimen:
Plasma and cellular components such as RBC, WBC, and platelets are present
Whole blood
Type of blood specimen:
With anticoagulant, requires immediate mixing following blood collection
Whole blood
Type of blood specimen:
Clear yellow supernatant on top upon settling of whole blood
Plasma
Type of blood specimen:
Obtained from a tube without anticoagulant
Serum
Which is clearer, plasma or serum?
Serum
T/F: the blood sample for serum does not require clotting before centrifugation, since the centrifugation will be able to separate its serum
F !
Settings for centrifugation of blood sample
1000-2000 g for 10 minutes
How much time is needed to clot a blood sample? (with and without clot activator)
1 hour (without)
30 minutes (with)
How much time is needed to clot a blood sample with thrombin?
5 minutes
In what test is gel activator discouraged due to its false absorption?
Therapeutic drug monitoring (absorbs drugs)
How much time is needed for the IV to be turned off before venipuncture on the site?
2 minutes
T/F: collection above the IV site is allowed
F (should be below)
This specific anticoagulant is used for lactose testing, glucose, and ethanol testing
Potassium oxalate (in gray tube)
This is the anticoagulant used for anticoagulant therapy and synovial fluid crystal analysis
Sodium heparin
This anticoagulant inhibits DNA polymerases
Heparin
This tube is used for lead testing
Tan colored
This anticoagulant causes blue background in wright’s staining
Heparin
This anticoagulant distorts cell morphology
Oxalate & Fluoride
This anticoagulant preserves labile coagulation factors
Citrate
This anticoagulant stimulates acid phosphatase
Citrate
Analytes increased with the use of tourniquet
Potassium, Albumin, Lactase, Ammonia
Alternative disinfectant for alcohol during alcohol testing
Benzalkonium chloride
Traces of alcohol causes what effect to the blood collected?
Hemolysis
T/F: rimming/ringing is done for all blood samples to ensure that centrifugation will have its optimal effect
F (serum samples only)
Rapid swelling at or near the venipuncture site due to blood leaking into the tissues
Hematoma
Anemia as a result of treatment
Iatrogenic anemia
The processing of a sample should be done within __ hours
4
The refrigeration setting for samples should be at __ for __ hours
4C
8 hours
The freezing setting for samples should be at __ for longer periods, if later testing is required
-20C