02 AMMONIA Flashcards
A nonprotein nitrogen that is produced from the deamination of amino acid
Ammonia
Ammonia is consumed by the parenchymal cells of the liver in the production of ___
urea
Most ammonia in the blood exists as ___ excreted by the kidneys and acts as to buffer urine
Ammonium ion (NH4+)
A condition with increased ammonia that occurs most commonly in children due to aspirin administration
Reye’s syndrome
Enzyme defect in Hyperammonemia type-I
Carbamoyl phosphate synthetase I
Enzyme defect in Hyperammonemia type-II
Ornithine Transcarba-moylase
Enzyme defect in Citrullinemia Type I
Argino succinate synthetase
Enzyme defect in Argonisuccinic aciduria
Arginosuccinate lyase
Enzyme defect in Hyperagininemia
Arginase
Enzyme defect in Citrullinemia type-II
Citrin
Enzyme defect in HHH syndrome
Ornithine transporter defect
High concentrations of ammonia are ___ and often associated with ___ ___
neurotoxic
hepatic encephalopathy
This method of ammonia determination exploited the volatility of ammonia to separate the compound in microdiffusion chamber
Conway method
___ method measures the amount of ammonia determined by titration
Conway method
Most common ammonia determination method on automated instruments
Enzymatic method catalyzed by GLDH
The cation exchange resin used for ammonia determination is quantified by ___ reaction
Berthelot’s
During ammonia determination, venous blood should by placed on ___ immediately
ice
Suitable anticoagulants for ammonia determination
heparin and EDTA
In ammonia determination, samples should be centrifuged at ___ to ___C within ___ minutes of collection
0-4
20
In ammonia determination, frozen plasma is stable for several days at ___C
-20
In ammonia determination, hemolysis should be avoided as RBCs contain ___ times as much ammonia as plasma
2-3
It is recommended that px. do not smoke for several hours before ammonia determination, as this will falsely ___ the results
increase
Normal range of plasma ammonia in adult
19-60 ug/dL
Normal range of urine ammonia in adult
140-1500 mg N/day
Normal range of plasma ammonia in children
68-136 ug/dL
Conversion factor of ammonia from conventional to SI unit
0.587
