02 AMMONIA

Question 1

A nonprotein nitrogen that is produced from the deamination of amino acid

Answer 1

Ammonia

Question 2

Ammonia is consumed by the parenchymal cells of the liver in the production of ___

Answer 2

urea

Question 3

Most ammonia in the blood exists as ___ excreted by the kidneys and acts as to buffer urine

Answer 3

Ammonium ion (NH4+)

Question 4

A condition with increased ammonia that occurs most commonly in children due to aspirin administration

Answer 4

Reye’s syndrome

Question 5

Enzyme defect in Hyperammonemia type-I

Answer 5

Carbamoyl phosphate synthetase I

Question 6

Enzyme defect in Hyperammonemia type-II

Answer 6

Ornithine Transcarba-moylase

Question 7

Enzyme defect in Citrullinemia Type I

Answer 7

Argino succinate synthetase

Question 8

Enzyme defect in Argonisuccinic aciduria

Answer 8

Arginosuccinate lyase

Question 9

Enzyme defect in Hyperagininemia

Answer 9

Arginase

Question 10

Enzyme defect in Citrullinemia type-II

Answer 10

Citrin

Question 11

Enzyme defect in HHH syndrome

Answer 11

Ornithine transporter defect

Question 12

High concentrations of ammonia are ___ and often associated with ___ ___

Answer 12

neurotoxic
hepatic encephalopathy

Question 13

This method of ammonia determination exploited the volatility of ammonia to separate the compound in microdiffusion chamber

Answer 13

Conway method

Question 14

___ method measures the amount of ammonia determined by titration

Answer 14

Conway method

Question 15

Most common ammonia determination method on automated instruments

Answer 15

Enzymatic method catalyzed by GLDH

Question 16

The cation exchange resin used for ammonia determination is quantified by ___ reaction

Answer 16

Berthelot’s

Question 17

During ammonia determination, venous blood should by placed on ___ immediately

Answer 17

ice

Question 18

Suitable anticoagulants for ammonia determination

Answer 18

heparin and EDTA

Question 19

In ammonia determination, samples should be centrifuged at ___ to ___C within ___ minutes of collection

Answer 19

0-4
20

Question 20

In ammonia determination, frozen plasma is stable for several days at ___C

Answer 20

-20

Question 21

In ammonia determination, hemolysis should be avoided as RBCs contain ___ times as much ammonia as plasma

Answer 21

2-3

Question 22

It is recommended that px. do not smoke for several hours before ammonia determination, as this will falsely ___ the results

Answer 22

increase

Question 23

Normal range of plasma ammonia in adult

Answer 23

19-60 ug/dL

Question 24

Normal range of urine ammonia in adult

Answer 24

140-1500 mg N/day

Question 25

Normal range of plasma ammonia in children

Answer 25

68-136 ug/dL

Question 26

Conversion factor of ammonia from conventional to SI unit

Answer 26

0.587