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Neville and Damm > What's my stain, what's my stain > Flashcards

What's my stain, what's my stain Flashcards

1
Q

Alveolar soft part sarcoma

A

TFE3 +, transcription factor PAS-D+ rhomboid crystals, t(X,17), ASPL-TFE3 fusion gene

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2
Q

Synovial sarcoma

A

TLE1, EMA, CK7 (epithelial and spindle component), t(X,18), SSX-SS18 fusion gene

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3
Q

Solitary fibrous tumor

A

STAT6 + BCL2 + CD34 + CD99+

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4
Q

Myofibroma

A

SMA+ MSA+ Desmin -

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5
Q

Paraganglioma

A

Chromo +, synapto + main tumor cells Sustentacular cells: s100+ ***same as a pheochromocytoma- these tumor are only different because paraganglioma is outside adrenal and pheochromcytoma is inside the adrenal medulla

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6
Q

Granular cell tumor

A

S100+, CD68+

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7
Q

Congenital epulis

A

S100-

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8
Q

Hemangioma of infancy

A

GLUT1 +

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9
Q

Leiomyoma

A

MSA +, SMA +, desmin +

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10
Q

Liposarcoma

A

MDM2 +, CDK4+….FISH to see amplification. IHC for these is less specific, especailly when done separate. MDM2 and CDK4 IHC together is more specific but less sensitive than MDM2 IHC alone

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11
Q

Angiosarcoma

A

Factor 8 +, CD31 +, CD34+ FLI-1 +, ERG +

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12
Q

Kaposi sarcoma

A

HHV 8 + aka LANA-1 + (protein encoded by HHV8) ERG+ CD34+ CD31 + (some but not all) FLI-1 + (so HHV8 differentiates from angiosarc)

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13
Q

Rosai-Dorfman Disease

A

CD68+ CD163+ S100 + CD1a -

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14
Q

Secretory Carcinoma

A

Secretory Material: PAS Alcean Blue Mammaglobin Tumor cells Mammaglobin S100 STAT5a BRST2 (aka GCDFP15) CKs Myoepithelial markers are negative Molecular: t(12,15) ETV6-NTRK3

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15
Q

Neurofibroma

A

S100 + CD34 +

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16
Q

Pleomorphic Adenoma

A

Myoepithelial markers: p63 + SMA + Epithelial markers: CAM5.2 CK7 Genetic mutation: PLAG1 + (IHC or ISH) Neuro / Dendritic markers: SOX10 + GFAP +

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17
Q

Melanoma

A

S100+ (spindle cell melanoma retains) Melan-A+ HMB45 + SOX10 + (spindle cell melanoma retains) Tyrosinase + MITF+

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18
Q

Small Round Blue Cell Tumor

A

MR SLEEP Melanoma (S100,SOX10,HMB45,Melan-A, etc) Rhabdomyosarcoma(Desmin,Myogenin, MyoD1) Sinonasal Undiff Ca (SNUC) (AE1/AE3, PanCK) Lymphoma (CD45, CD20, CD3) Ethesioneuroblastoma (olfactory) (S100, Synapto,Chromo,) Ewing Sarcoma (CD99, SMARCB1, FLI-1) Pituitary Adenoma (EGFR, Synapto,Chromo, Negative for most hormone markers (TSH,ACTH,LH,GH,FSH))

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19
Q

Langerhans Cell Histiocytosis

A

aka: eosinophilic granuloma (solitary) CD1a + S100 + Langerin + (AKA CD207)

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20
Q

Rosai-Dorfman

A

Aka: LN sinus histiocytosis with massive LAD CD68+ S100 + CD163 + CD1a - (emperipoesis on histo)

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21
Q

Osteosarcoma

A

MDM2 amplification on FISH

SATB2+ IHC

MDM2 and CDK4 coexpression (IHC or FISH amplification) to help diagnose low grade osteosarcomas and high grade osteosarcomas that progressed from low grade osteosarcomas

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22
Q

Plasma cell neoplasm

A

CD138+, CD79a+, Kappa / Lamda ISH

(since you always need a reminder: histiocytes are cd68, cd163)

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23
Q

Ectomesenchymal Chondromyxoid tumor

A

GFAP +

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24
Q

Granulomatous inflammation

A

AFB, GMS, PAS

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25
DIF Patterns: Lupus Erythematosus
SHAGGY / GRANULAR band at basement membrane zone with IgM, IgG, C3
26
IIF Pattern: Wegner Granulomatosis
PR3-ANCA (Proteinase-3 antineutrophil cytoplasm antibodies, prev c-ANCA) and MPO-ANCA (myeloperoxidase, prev p-ANCA) directed against cytoplasmic components of neutrophils
27
IIF Pattern: Lichenoid drug reactions
string of pearls (annular pattern) along cell membrane of the basal cell layer with IgG
28
DIF Patterns: Pemphigus Vulgaris
"Chicken wire" IgG, IgM, C3 in intercellular spaces of epithelial cells
29
DIF Patterns: Paraneoplastic pemphigus
weakly positive IgG, C3 intercellular zones of the epithelium AND/OR a linear deposition at the basement membrane zone
30
DIF Patterns: Mucous membrane pemphigoid
linear band of immunoreactants )IgG, C3 at the basement membrane zone
31
DIF Patterns: Bullous pemphigoid
Continuous linear band of immunoreactants (IgG, C3) localized to the basement membrane zone
32
DIF Patterns: Erythema multiforme
Non-diagnostic (granular C3 + around blood vessels)
33
DIF Patterns: Lichen Planus
Fibrinogen basement membrane zone
34
DIF: Chronic Ulcerative Stomatitis
"speckled" IgG in the basal and parabasal nuclei
35
Ewing sarcoma
CD99, FLI-1, SMARCB1
36
Acinic Cell Carcinoma
DOG1, SOX10, PASD+ , NR4A3+ (due to t(4,9))
37
Adenoid Cystic Carcnoma
Biphasic (ducal and myoepithelial): Epithelial Markers: CAM5.2, CK7 Myoepithelial Markers: P63+, P40+ Others: CKIT
38
Polymorphous Adenocarcinoma
Monophasic (ducal proliferation only): Epithelial Markers: CAM5.2, CK7 Myoepithelial markers: P63+, P40-
39
Malignant peripheral nerve sheath tumor
S100, SOX10
40
CEOT
Congo red +, apple green birefringence
41
Nodular Faciitis
MYH9-USP6 gene fusion SMA+ MSA+ Calponin + S100 - Desmin -
42
Pemphigus Vulgaris: "Chicken wire" IgG, IgM, C3 in intercellular spaces of epithelial cells
43
Pemphigus Vulgaris "Chicken wire" IgG, IgM, C3 in intercellular spaces of epithelial cells
44
Mucous membrane pemphigoid linear band of immunoreactants )IgG, C3 at the basement membrane zone
45
Mucous membrane pemphigoid linear band of immunoreactants )IgG, C3 at the basement membrane zone
46
Erythema multiforme Non-diagnostic (granular C3 + around blood vessels) Regezi: "EM: non-specific fine granular basement membrane zone fluorescence for C3"
47
Lichen Planus Fibrinogen highlighted in basement membrane zone Regezi: "BMZ and supericial LP fluor for fibrinogen and apoptotic body fluorescence for IgM"
48
Lichen Planus Fibrinogen (shaggy?), basement membrane zone Regezi: "BMZ and supericial LP fluor for fibrinogen and apoptotic body fluorescence for IgM"
49
Lupus Erythematosus SHAGGY / GRANULAR band at basement membrane zone with IgM, IgG, C3 Regezi :"course-granular BMZ for IgM"
50
Metastatic Renal Cell Carcinoma
PAX 8+, CD10 +, CAIX +
51
Metastatic Prostate Adenocarcinoma
FOXA1+, HSD3B2+, ROCK1, AMACR, PSA, PAP, NKX3
52
Metastatic Breast, Invasive Ductal Carcinoma
MUC4, EMA, CK7, ER/PR, GATA3
53
Metastatic Lung Adenocarcinoma
CK7, NAPSIN-A, TTF-1
54
Metastatic Thyroid Carcinoma
TTF-1, Thyroglobulin, PAX8
55
Metastatic Colon Adenocarcinoma
CDX2, CK20, AMACR, CD10 (stromal cells)
56
Merkel Cell Carcinoma
CK20 (classic dot-like perinuclear), MCPyV, Chromo/Synapto, CD56
57
Myopericytoma
SMA + h-caldesmon + rare desmin
58
Myofibroma
muscle specific actin (MSA/HHF35) + SMA + Desmin - pan-actin +
59
Inflammatory myofibroblastic tumor
POsitive: SMA, alpha smooth muscle actin, calponin, ALK
60
Glioma
GFAP
61
Fibrous Histiocytoma
CD163, CD168, FactorXIIIa
62
Histiocyte markers
CD68, CD163, CD168
63
Glomus Tumor
Positive for SMA, MSA, calponin, h-caldesmon, collagen type IV; negative for cytokeratin and S100

Neville and Damm (72 decks)

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